Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (14): 2563-2566.doi: 10.3969/j.issn.1673-8225.2012.14.019

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Effects of trypsogen and collagenase on culture of neural stem cells of fetal rat telencephalon  

Liang Jun1, Song Xiao-yu2, Zhao Fu-sheng1, Li Yue-zhen1, Dong Jian-jiang1   

  1. 1Department of Histology and Embryology, Mudanjiang Medical College, Mudanjiang  157011, Heilongjiang Province, China; 2Mudanjiang Cardiovascular Hospital, Mudanjiang  157011, Heilongjiang Province, China
  • Received:2011-10-06 Revised:2011-11-12 Online:2012-04-01 Published:2012-04-01
  • Contact: author: Dong Jian-jiang, Master, Lecturer, Department of Histology and Embryology, Mudanjiang Medical College, Mudanjiang 157011,Heilongjiang Province, China dongjj101@163.com
  • About author:Liang Jun☆, Studying for doctorate, Associate professor, Department of Histology and Embryology, Mudanjiang Medical College, Mudanjiang 157011, Heilongjiang Province, China
  • Supported by:

    the Young Key Scholars Project of Heilongjiang Com-advanced School, No.1155G60*

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Abstract:

BACKGROUND: Neural stem cells (NSCs) bring wide prospect for the study of neural development and repairing, and the in vitro culture is foundational to neuroscience.
OBJECTIVE: To contrast the effects of trypsogen and collagenase on culture of NSCs in vitro.
METHODS: The fetal rat telencephalons were isolated from SD rats pregnant for 14 days under sterile conditions, and tissues were divisively treated with trypsogen and typeⅠ collagenase contained EDTA following trituration, and cultured with serum-free medium.
RESULTS AND CONCLUSION: The adherent monoculture NSCs were obtained using collagenase and congeries of cells were obtained using trypsogen. Nestin immunofluorescence identification showed the cells were positive, and the purity was over 99%. The primary adherent monoculture NSCs can be simply and quickly got using collagenase.
 

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