Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (1): 39-42.doi: 10.3969/j.issn.1673-8225.2012.01.008

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Osteoblasts derived from human embryonic stem cells by CD271 magnetic beads in vitro

Jiang Hong-hong1, Zhang Jin-li2, Li Fu-gui3, Wang Tao3   

  1. 1Department of Ultrasound, Guangzhou Red Cross Hospital, Guangzhou,  510220, Guangdong Province, China; 2Institute of Traumatic Surgery, Guangzhou Red Cross Hospital, Guangzhou  510220, Guangdong Province, China; 3Center for Stem Cell Biology and Tissue Engineering, Sun Yat-sen University, Guangzhou,  510080, Guangdong Province, China
  • Received:2011-05-20 Revised:2011-08-02 Online:2012-01-01 Published:2012-01-01
  • Contact: Wang Tao, Doctor, Supervising technician, Center for Stem Cell Biology and Tissue Engineering, Sun Yat-sen University, Guangzhou, 510080, Guangdong Province, China wangt69@mail.sysu.edu.cn
  • About author:Jiang Hong-hong★, Master, Department of Ultrasound, Guangzhou Red Cross Hospital, Guangzhou, 510220, Guangdong Province, Chinajhhnumber1@163.com
  • Supported by:

     the National Natural Science Foundation of China, No.81000362*

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Abstract:

BACKGROUND: Studies on osteoblasts derived from human embryonic stem cells (hESCs) had made remarkable results, but the induced methods can produce a great number of effective osteoblasts derived from hESCs without other miscellaneous cells is not established.
OBJECTIVE: To explore the feasibility of osteoblasts derived from hESCs in vitro by using CD271 magnetic beads sorting system.
METHODS: CD271 positive cells were obtained from hESCs lines SYSU-2 cells through embryoid bodies formation at 6 days and attachment differentiation at 14 days by using CD271 magnetic beads sorting technology. Cell surface antigen expression, karyotype analysis and potential of differentiation were detected on CD271 positive cells.
RESULTS AND CONCLUSION: CD271 positive cells exhibited regular spindle shape, which was similar to morphology of bone marrow-derived mesenchymal stem cells (BMSCs), and could be cultured in vitro about 14 generation remaining stable karyotype. At the same time, these cells expressed similar cell surface antigen marks (CD45 negative; CD73, CD105 and CD166 positive) with BMSCs. But differentiation potential identification showed that CD271 positive cells were solely induced osteoblasts formation. It has tremendous significance to study for the bone development mechanism and supply with enough, safe and efficient seed cells for bone tissue engineering.

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