Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (1): 35-38.doi: 10.3969/j.issn.1673-8225.2012.01.007

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Characteristics of fetal non-adherent bone marrow stromal cells 

Xiao Long-yan1, Fu Jin-xiang1, Zhang Xue-guang2, Chen Qiu2, Zhang Hong1, Wang Pan-jun1, Sun Yu1, Wang Ming-yuan3,
Gu Yan-zheng2
 
  

  1. 1Second Affiliated Hospital of Soochow University, Suzhou  215004, Jiangsu Province, China; 2Institute of Biotechnology of Soochow University, Suzhou  215007, Jiangsu Province, China; 3Suzhou Red Cross Blood Centre, Suzhou  215006, Jiangsu Province, China
  • Received:2011-08-15 Revised:2011-11-24 Online:2012-01-01 Published:2012-01-01
  • Contact: Fu Jin-xiang, Professor, Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu Province, China uufjxly@public1.sz.js.cn
  • About author:Xiao Long-yan★, Studying for master’s degree, Second Affiliated Hospital of Soochow University, Suzhou 215004, Jiangsu Province, China Xiaolongyan1984@sina.com
  • Supported by:

    the National Natural Science Foundation of China, No. 81071943*

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Abstract:

Abstract
BACKGROUND: There are non-adhesion stem cells in bone marrow stem cells, and the morphology and phenotype characteristics of the cells are similar to adhesion bone marrow mesenchymal stem cells.  
OBJECTIVE: To explore the characteristics of fetal non-adherent bone marrow stromal cells (NA-BMSCs).
METHODS: The fetal NA-BMSCs were cultivated by the method of adherent separation repeatly (“pull-offs”), the suspended cells were incubated in another culture bottle every 24 hours for 4 times. The growth curve of the passage 1, 3, 5 NA-BMSCs was detected by MTT assay, and the phenotype of the NA-BMSCs was determined with flow cytometry. Passage 1 NA-BMSCs were preformed with hematopoietic colony assay and passage 3 NA-BMSCs were preformed with osteogenic and adipogenic induction.
RESULTS AND CONCLUSION: After four times’ “pull-off”, there were still asherent cells and colony formation; the resulting cells were continuing to culture for 7 days and those cells were the primary NA-MSCs (P0). The morphology of the cells was uneven; they highly expressed the integrin and the surface markers of endothelial cells with a middle level of CD106. Interestingly, they also expressed the surface markers of hematopoietic cells CD34. Those cells had multi-differentiation potency in vitro and they could differentiate into osteoblasts and hematopoietic cells under different inducing medium, but the capability of lipid differentiation was weak.

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