Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (5): 818-822.doi: 10.3969/j.issn.1673-8225.2011.05.014

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Early diagnostic value of heat shock protein 70 in acute rejection after liver transplantation in rhesus monkeys

Li Zhu, Ran Jiang-hua, Liu Jing, Zhang Sheng-ning, Wu Shu-yuan, Liang Yu, Zhang Xi-bing, Li Li   

  1. First Endemic Area of Liver, Gall and Pancreas, Kunming First People’s Hospital, Ganmei Hospital Affiliated to Kunming Medical University, Kunming   650011, Yunnan Province, China
  • Received:2010-11-10 Revised:2010-12-03 Online:2011-01-29 Published:2011-01-29
  • Contact: Ran Jiang-hua, Doctor, Chief physician, Professor, Master’s supervisor, First Endemic Area of Liver, Gall and Pancreas, Kunming First People’s Hospital, Ganmei Hospital Affiliated to Kunming Medical University, Kunming 650011, Yunnan Province, China rjh2u@163.com
  • About author:Li Zhu☆, Doctor, Attending physician, First Endemic Area of Liver, Gall and Pancreas, Kunming First People’s Hospital, Ganmei Hospital Affiliated to Kunming Medical University, Kunming 650011, Yunnan Province, China lllllzhu@126.com
  • Supported by:

    Major Program of Kunming Science and Technology Bureau, No.08S100304*

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Abstract:

BACKGROUND: Heat shock protein 70 (HSP 70) is a special kind of protein by living organisms stress synthesized under the adverse environmental stimulation. It takes part and plays an important role in transplantation immunoreactions.
OBJECTIVE: To investigate the early diagnostic value HSP 70 on acute rejection after liver transplantation in rhesus monkey.
METHODS: Healthy rhesus monkeys were selected. A total of 16 cases underwent allogeneic orthotopic liver transplantation with improved two-cuff and hepatic artery reconstruction methods, which were divided into acute rejection and control group (8 cases for each group). Immunosuppressive therapy was not applied in the acute rejection group, but was applied in the control group during recipients' perioperative period. Liver tissue after transplantion were obtained respectively at 6, 12, 24, 72 hours points to treated with hematoxylin-eosin (HE) staining, and then the degree of rejection was determined. The expression level of HSP 70 in liver tissue was detected by western blotting. The expression of HSP 70 in liver tissue was detected by immunohistochemistry.
RESULTS AND CONCLUSION: Histological changes of liver acute rejection in acute rejection group were more serious than that in control group. Banff grade level in acute rejection group was higher than that in control group (P < 0.05). The expression levels of HSP 70 in both groups of transplanted liver were increased by immunohistochemistry and western blotting detection from the beginning to 72 hours after transplantation. However, the level of HSP 70 in acute rejection group was significantly higher than that in control group (P < 0.05). It is indicated that the early acute rejection of rhesus monkeys at 72 hours after transplantation was significantly observed without immunosuppressive therapy. The expression level of HSP 70 presents an obvious rising trend with the incidence and progress of acute rejection. HSP 70 has a high prediction and diagnostic value on early acute rejection after liver transplantation.

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