Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (5): 788-791.doi: 10.3969/j.issn.1673-8225.2011.05.007

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Effects of perfusion speed on graft ischemia/reperfusion injury following liver transplantation

Xiao Hong, Yin Si-neng, Chen An-ping, Tian Gang, Chen Xian-lin, Long Fei-wu   

  1. Department of Hepatobiliary Surgery, the Second People’s Hospital of Chengdu, Chengdu 610017, Sichuan Province, China
  • Received:2010-08-13 Revised:2010-10-15 Online:2011-01-29 Published:2011-01-29
  • Contact: Long Fei-wu, Master, Attending physician, Department of Hepatobiliary Surgery, the Second People’s Hospital of Chengdu, Chengdu 610017, Sichuan Province, China longfw1978@sina. com
  • About author:Xiao Hong, Associate chief physician, Department of Hepatobiliary Surgery, the Second People’s Hospital of Chengdu, Chengdu 610017, Sichuan Province, China
  • Supported by:

     the Science and Technology Foundation of Health Department of Sichuan Province, No. 080015*, 090025*

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Abstract:

BACKGROUND: Previous studies demonstrated that perfusion pressure influences the energy metabolism of graft and affects its energy, suitable perfusion pressure can significantly improve the quality of donor.
OBJECTIVE: To investigate the effects of various perfusion-speeds on reperfusion injury of graft after rat orthotopic liver transplantation.
METHODS: SD to SD rat orthotopic liver transplantation models were established by improved Kamada two-cuff technique. Perfusion-speed during harvesting grafts was 50, 100, 150 and 200 mL/h, respectively. The morphological change of grafts were observed by light microscope, alanine transarninase (ALT) and tumor necrosis factor-α (TNF-α) in peripheral serum and endothelial nitric oxide synthase (eNOS) protein and mRNA intragraft were detected.
RESULTS AND CONCLUSION: The pathohistological damage of grafts in 200 mL/h group was obviously compared with other groups. Compared with 50 and 100 mL/h groups, ALT in 150 and 200 mL/h groups was significantly decreased (P < 0.05, P < 0.01), but the expression of eNOS protein and mRNA was obviously lower (P < 0.01). The hepatic function damaged seriously with increasing of perfusion speed after 100 mL/h. The findings demonstrated that suitable perfusion speed can reduce hepatic function injury and improve prognosis. 100 mL/h was compatible speed for harvesting graft of rat liver transplantation.

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