Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (47): 8782-8786.doi: 10.3969/j.issn.1673-8225.2011.47.011

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Inflammatory response evaluation for electrospun polycaprolactone/silk sericin membranes in vivo

Lü Yong-gang1,2, Lin Chong-wen1,2, Li Lin-hao1,2, Qian Yu-na1,2, Zhang Xiao-mei1,2, Yang Li1,2, He Jian-hua1,2   

  1. 1Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing  400044, China
    2“111” Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing  400044, China
  • Received:2011-08-03 Revised:2011-10-20 Online:2011-11-19 Published:2011-11-19
  • About author:Lü Yong-gang☆, Doctor, Professor, Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400044, China; “111” Project Laboratory of Biomechanics and Tissue Repair, Bioengineering College, Chongqing University, Chongqing 400044, China yglv@cqu.edu.cn
  • Supported by:

    the Program for New Century Excellent Talents in University, No. NCET-10-0879*; the Key Science and Technology Program of Chongqing Science and Technology Commission, No. 2009AA5045*; the National Natural Science Foundation of China, No. 11172338*; the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry*; the Sharing Fund of Chongqing University's Large-scale Equipment* 

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Abstract:

BACKGROUND: Studies have shown that silk sericin (SS) can accelerate the proliferation of mammalian cells and promote wound healing. SS has good activities for antibacteria, antioxidation and anticancer. SS also has a good biocompatibility and biodegradability. It is an ideal material for tissue engineering, however, there is still a controversy of whether SS causes inflammatory response so far.
OBJECTIVE: To evaluate the inflammatory response of electrospun polycaprolactone (PCL)/SS membranes.
METODS: Materials with different PCL/SS ratios were implanted into the back muscles of rats and were taken out after 4 weeks. The inflammatory response was evaluated by hematoxylin eosin staining and macrophage antibody CD68 immunohistochemistry.
RESULTS AND CONCLUSION: The inflammatory response of the 7:3 group of PCL/SS membranes is similar to that of silk fibroin membrane and was less than those of the 6:4, 5:5 and PCL groups. The 6:4 and 5:5 groups have similar inflammatory response with PCL group. Electrospun PCL/SS membranes can not cause significant inflammatory response. A little amount of SS can decrease the inflammatory response of PCL membrane. However, the inflammatory response of PCL/SS membrane can increase as the ratio of SS to PCL increased.

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