Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (47): 8777-8781.doi: 10.3969/j.issn.1673-8225.2011.47.010

Previous Articles     Next Articles

Role of adipose-derived mesenchynal stem cell combined with silk fibroin scaffold in enhancing vascular formation in rabbit urethral repair

Zhang Ya1, Zhou Yun1, Jia Li-shan1, Zhai Jing-mei1, Miao Xiao-fen2, Wang Rui2   

  1. 1Department of Surgery, Soochow University Children’s Hospital, Suzhou  215003, Jiangsu Province, China
    2Department of Pathology, Suzhou Traditional Chinese Medical Hospital, Suzhou  215003, Jiangsu Province, China
  • Received:2011-03-14 Revised:2011-05-13 Online:2011-11-19 Published:2011-11-19
  • Contact: Zhou Yun, Doctor, Professor, Chief physician, Department of Surgery, Soochow University Children’s Hospital, Suzhou 215003, Jiangsu Province, China pediaturology@ yahoo.com.cn
  • About author:Zhang Ya, Associate chief examiner, Department of Surgery, Soochow University Children’s Hospital, Suzhou 215003, Jiangsu Province, China zhangya62@ yahoo.com.cn
  • Supported by:

    the General Program of Jiangsu Provincial Health Bureau, No. H200819*; the Social Development Program of Suzhou City, No. SS08021*

PDF

384

Abstract:

BACKGROUND: We searched the relevant research papers on main domestic and foreign online retrieval databases in recent five years, and no publication was found on the uretral defect repair with adipose-derived mesenchymal stem cells (ADMSCs) on silk fibroin scaffold.
OBJECTIVE: To study the effect of ADMSCs combined with a porous silk fibroin scaffold (PSFS) on enhancing vascular formation in rabbit urethral repair.
METHODS: The ADMSCs were separated from the epididymal fat pads of the rabbits, and then treated with cell isolation and culture. In animal study, the rabbits were assigned into group A with the urethral defects, group B with urethral defects repaired by PSFS, and group C with urethral defects repaired by ADMSCs seeded on PSFS.
RESULTS AND CONCLUSION: The expression of CD44 and Vimentin was positive on ADMSCs of third passage by indirect immunoflurescence methods. On histological examination, blood vessel formation in group C was significantly more abundant than groups A and B in the 2nd to 6th week after operation. The FⅧ-RAg positive microvessel density was also significantly more than groups A and B in the 4th week after operation (P < 0.05). ADMSCs combined with silk fibroin scaffold can promote in vivo vascularization for urethral repair.

CLC Number: