Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (47): 8769-8772.doi: 10.3969/j.issn.1673-8225.2011.47.008

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In vitro construction of decellularized vascular xenograft by improved enzymatic method

Wu Xin, Gu Yong-quan, Duan Hong-yong, Chen Bing, Li Jian-xin, Wu Ying-feng, Zhang Shu-wen, Wang Zhong-gao, Zhang Jian   

  1. Department of Vascular Surgery, Xuanwu Hospital, Capital Medical University, Beijing  100053, China
  • Received:2011-04-19 Revised:2011-06-19 Online:2011-11-19 Published:2011-11-19
  • Contact: Gu Yong-quan, Doctor, Chief physician, Department of Vascular Surgery, Xuanwu Hospital, Capital Medical University, Beijing 100053, China
  • About author:Wu Xin☆, Doctor, Attending physician, Department of Vascular Surgery, Xuanwu Hospital, Capital Medical University, Beijing 100053, China wuxinbj@yahoo.cn
  • Supported by:

    the National 863 Program of China, No. 2006AA02A134*; the Natural Science Foundation of Beijing, No. 5082007*, 2103049*

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Abstract:

BACKGROUND: How to expand the pore size and porosity of the acellular matrix to facilitate the post-cell re-cultivation requirement, and simultaneously the graft must ensure its mechanical requirements, is one of the hotspots in vascular tissue engineering.
OBJECTIVE: To observe the mechanical properties and histocompatibility of decellularized vascular xenograft vascular grafts constructed by the improved enzymatic method.
METHODS: Porcine carotid arteries were decellularized using conventional trypsin-EDTA plus 1% TritonX-100 and 0.1% ammonia in order for 4, 5, and 6 hours.
RESULTS AND CONCLUSION: Porcine carotid arteries were decellularized by trypsin, ammonium hydroxide and non-ionic detergent to extract all the cell components of the grafts successfully. To obtain better pore size and porosity of the acellular scaffold, we changed the methods by extending the time of trypsin. By extending the time of trypsin methods, the better pore size and porosity of the acellular scaffold was obtained. Biomechanics, porosity characteristics of scaffold can meet the requirements of tissue engineered vessel.

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