Abstract:

BACKGROUND: Different islet transplantation sites in diabetic mice influence the success rate of islet transplantation.
OBJECTIVE: To explore a more ideal transplantation site by comparing the transplantation effect of lesser omentum, kidney capsule and axillary fossa.
METHODS: Lymphocytes separation medium was used to isolate and purify BACL/c mice islets, which were transplanted into C57BL/6 diabetic mice. Then the effect of different transplantation sites including lesser omentum, kidney capsule and axillary fossa was compared.
RESULTS AND CONCLUSION: After isolation and purification, about (102 ± 4) islets per mouse were obtained and possessed excellent activity. After islets were transplanted into lesser omentum, kidney capsule and axillary fossa, the blood glucose of diabetic mice was significantly lower than normal extent (< 14.00 mmol/L). The blood glucose had no significant difference between lesser omental and kidney capsule groups (P > 0.05), but was significantly lower compared with axillary fossa group (P < 0.05). At 7 days after islet transplantation, the islet shape of lesser omentum group was basically complete, the islet shape of the kidney capsule group was irregular, that of the axillary fossa group was destroyed and many inflammatory cells were found in the axillary fossa group. The lesser omentum and kidney capsule groups regulated blood glucose level better than the axillary fossa group. The lesser omentum possessed larger capacity and better blood supply. The lesser omentum can be used as an ideal site of islet transplantation.