Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (42): 7868-7872.doi: 10.3969/j.issn.1673-8225.2011.42.019

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Influence of polylactic/poly glycolic acid microspheres modified by collagen type I on the adhesion and osteogenic differentiation of bone marrow mesenchymal stem cells

Yu Zheng-rong1, Li Chun-de1, Shi Xu-dong2, Liu Xian-yi1, Sun Hao-lin1, Yang Xin1, Zhu Tian-yue1   

  1. 1Department of Orthopedics, Peking University First Hospital, Beijing  100034, China
    2Chemical Institute, Chinese Academy of Sciences, Beijing  100190, China
  • Received:2011-02-19 Revised:2011-04-19 Online:2011-10-15 Published:2011-10-15
  • Contact: Li Chun-de, Chief physician, Department of Orthopedics, Peking University First Hospital, Beijing 100034, China lichunde@medmaili.com.cn Correspondence to: Zhu Tian-yue, Doctor, Professor, Department of Orthopedics, Peking University First Hospital, Beijing 100034, China jointonline@263.net
  • About author:Yu Zheng-rong★, Master, Attending doctor, Department of Orthopedics, Peking University First Hospital, Beijing 100034, China dryzr@163.com

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Abstract:

BACKGROUND: To meet with the need of terminal cells’ ossification function in bone tissue engineering, bone marrow mesenchymal stem cells need to be induced with DMEM in vitro or with techniques such as gene transfection in vivo.
OBJECTIVE: To investigate the adhesion and osteogenic differentiation capacity of bone marrow mesenchymal stem cells on polylactic/poly glycolic acid (PLGA) microspheres stents modified by Collagen type I.
METHODS: PLGA microspheres stents were prepared, and bone marrow mesenchymal stem cells were isolated and purified from female SD rats. The third passage bone marrow mesenchymal stem cells were cocultured with untreated PLGA microspheres or those modified by collagen type I for 14 days. Cell adhesion and growth on different stents were observed.
RESULTS AND CONCLUSION: Scanning electronic microscope and FDA-PI staining showed bone marrow mesenchymal stem cells could grow on PLGA microspheres stents. Compared with those on untreated PLGA microspheres, bone marrow mesenchymal stem cells were more prone to proliferate on and adhesive to PLGA microspheres modified with collagen type I. PLGA microspheres modified with collagen type I help the adhesion and proliferation of bone marrow mesenchymal stem cells, and have a certain ability to induce the osteogenic differentiation of bone marrow mesenchymal stem cells.

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