Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (42): 7873-7876.doi: 10.3969/j.issn.1673-8225.2011.42.020

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Influence of recombinant human bone morphogenetic protein-2 loaded microspheres combined with fibrin glue on the proliferation and differentiation of canine bone marrow stromal cells

Yao Qi1, Zhang Li-hai2, Huang Peng2, Cui Geng2, Tang Pei-fu2   

  1. 1Department of Orthopedics, Beijing Shijitan Hospital, Capital Medical University, Beijing  100038, China
    2Department of Orthopedics, General Hospital of Chinese PLA, Beijing  100853, China
  • Received:2011-03-28 Revised:2011-04-30 Online:2011-10-15 Published:2011-10-15
  • About author:Yao Qi, Doctor☆, Associate chief physician, Department of Orthopedics, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China yqjh2005@gmail.com
  • Supported by:

    the National Natural Science Foundation of China, No. 81000796*; the Natural Science Foundation of Beijing, No. 7103170*; Beijing Excellent Talent Fund, No. 2010D003034000040*; Youth Science Research Fund of Beijing Health Bureau, No. QN 2009-017*

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Abstract:

BACKGROUND: It has been proved that poly (lactic-co-glycolic) acid (PLGA) microsphere/fibrin glue (FS) has satisfactory slow-release effect as recombinant human bone morphogenetic protein-2 (rhBMP-2) carrier.
OBJECTIVE: To investigate the influence of injectable BMP release system by combining rhBMP-2/PLGA microsphere with FS on the proliferation and differentiation of canine bone marrow stromal cells (BMSCs).
METHODS: The rhBMP-2/PLGA microsphere was prepared using W/O/W solvent evaporation methods. rhBMP-2 loaded PLGA microsphere were incorporated in FS to establish injectable BMP release system. The influence of injectable BMP release system on BMSCs proliferation and differentiation were investigated by histochemistry method and cell culture.
RESULTS AND CONCLUSION: Inhibition of BMSCs proliferation did not occur in response to in vitro culture with the rhBMP-2/PLGA microsphere/FS composite, but the differentiation of BMSCs was increased. rhBMP-2/PLGA microsphere/FS composite has satisfactory effect on improving in vitro osteogenic potential of BMSC as a good carrier of BMP.

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