Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (40): 7475-7479.doi: 10.3969/j.issn.1673-8225.2011.40.015

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Application of cycling perfusion seeding method in bone tissue engineering of human umbilical cord derived mesenchymal stem cells

Li Min1,2, Li Wei-wei2, Yang Bing3, Li Xiao-gang1, Qi Nian-min2   

  1. 1School of Life Science, Lanzhou University, Lanzhou  730000, Gansu Province, China
    2Cell Engineering Laboratory of Biomedicine, School of Pharmacy, Shanghai Jiao Tong University, Shanghai  200240, China
    3Jiangsu Public Stem Cell Engineering Technology Research Center, Taizhou  225300, Jiangsu Province, China
  • Received:2011-01-05 Revised:2011-03-18 Online:2011-10-01 Published:2011-10-01
  • Contact: Li Wei-wei, Studying for doctorate, Cell Engineering Laboratory of Biomedicine, School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China liweiwei1984@sjtu.edu.cn
  • About author:Li Min★, Studying for master’s degree, School of Life Science, Lanzhou University, Lanzhou 730000, Gansu Province, China; Cell Engineering Laboratory of Biomedicine, School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China lm198110@sina.cn
  • Supported by:

    the National Science & Technology Major Project of China (Key Innovative Drug Development), No.2009ZX09306-
    008*

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Abstract:

BACKGROUND: A set of modular three-dimensional perfusion bioreactor system was pre-designed in the previous studies, which has be initially applied for implantation of rat bone marrow mesenchymal stem cells into a three-dimensional non-woven polyethylene terephthalate (PET) fiber sheet carrier in bone tissue engineering.
OBJECTIVE: To investigate the use of self-made cycling perfusion seeding method for human umbilical cord derived mesenchymal stem cells (hUCMSCs) seeded on PET sheet carrier.
METHODS: Primary hUCMSCs were cultured and passaged. The fifth passage of cells were characterized by determining their surface antigen using a flow cytometry and then seeded onto three-dimentional PET disc scaffolds by static seeding method and cycling perfusion seeding method (low perfusion rate and high perfusion rate).
RESULTS AND CONCLUSION: The hUCMSCs of the fifth passage were steady in the cell shapes, with good viability and highly expressed CD90, CD105, while were negative for CD14, CD45. The cycling perfusion seeding method was superior to the static seeding method in the seeding efficiency, cell density and proliferative ability. Dehydrogenase activity and delayed period of cells seeded by using the cycling perfusion seeding method with high perfusion rate were better than those by using the static seeding method. Alkaline phosphatase activity was also higher in the cycling perfusion seeding method group than the static seeding method. The data suggested that the cycling perfusion seeding method is more suitable for hUCMSCs bone tissue engineering and further optimization of this method is needed.

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