Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (32): 6012-6015.doi: 10.3969/j.issn.1673-8225.2011.32.028

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Effect of olfactory ensheathing cell transplantation on gene expression of Sema3A and its receptor, NP-1, in rats following spinal cord injury

Zhu Zhen-dong1, Zhang Lin2, Zhou Xue3   

  1. 1Department of Human Anatomy, 2Department of Forensic Biology, Xinxiang Medical College, Xinxiang  453003, Henan Province, China; 3Department of Histology, Embryology and Neurobiology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu  6l0041, Sichuan Province, China
  • Received:2011-01-22 Revised:2011-03-14 Online:2011-08-06 Published:2011-08-06
  • Contact: Zhou Xue, Doctor, Professor, Department of Histology, Embryology and Neurobiology, West China School of Preclinica1 and Forensic Medicine, Sichuan University, Chengdu 6l0041, Sichuan Province, China zhouxuel@mail.sc.cninfo.net
  • About author:Zhu Zhen-dong★, Master, Assistant teacher, Department of Human Anatomy, Xinxiang Medical College, Xinxiang 453003, Henan Province, China Stay0413@163.com

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Abstract:

BACKGROUND: Studies have proved that olfactory ensheathing cells (OECs) can stimulate the repair of spinal cord injury, but the research for mechanism of nerve growth inhibition guidance factor is rarely reported.
OBJECTIVE: To investigate the effects of OECs transplantation on the change of mRNA of Sema3A and NP-1.
METHODS: A total of 17 female adult healthy Sprague-Dawley rats were randomly divided into 3 groups: OECs transplanted group, DMEM/F12 transplanted group and normal control group. Transplanted groups were anaesthetized and then were acutely transplanted after the left spinal cord hemisection on the level of T11-12 with suspension of OECs or DMEM/F12 culture fluid.
RESULTS AND CONCLUSION: Two segments of the spinal cord adjacent to the hemisected segments were dissected 6 weeks later, and reverse transcription polymerase chain reaction (RT-PCR) was used for semi-quantitative analysis of the mRNA of Sema3A and NP-1. There were a large number of OECs alive 6 weeks later. The mRNA quantity of Sema3A and NP-1 in the DMEM/F12 transplanted group was more than that in the normal control group (P < 0.05). But the mRNA quantity of Sema3A and NP-1 in the OECs transplanted group was markedly down-regulated compared with the DMEM/F12 transplanted group, and there was no significant difference between OECs transplanted group and normal control group (P > 0.05). These indicated that the mRNA expression of Sema3A and NP-1 was effectively decreased by OECs transplantation.

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