Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (16): 2889-2894.doi: 10.3969/j.issn.1673-8225.2011.16.010

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Propylene carbonate/chitosan nanofiber porous scaffolds composited with bone marrow mesenchymal stem cell for bone defect repair in rabbits

Huan Song-wei1,2, Zha Zhen-gang1,2, Yao Ping3, Wu Hao1,2, Liu Ning1,2, Zhao Jian-hao4, Xiong Gao-xin1, She Guo-rong1,2   

  1. 1Institute of Orthopedic Diseases, 2Department of Orthopedics, First Affiliated Hospital of Medical College, 3Department of Physiology, Medical College, 4Department of Materials Science and Engineering, College of Sciences and Engineering, Jinan University, Guangzhou  510630, Guangdong Province, China
  • Received:2011-01-12 Revised:2011-03-18 Online:2011-04-16 Published:2013-11-11
  • Contact: Zha Zhen-gang, Professor, Chief physician, Doctoral supervisor, Institute of Orthopedic Diseases, Jinan University, Guangzhou 510630, Guangdong Province, China; Department of Orthopedics, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China pingsha2008@sohu.com
  • About author:Huan Song-wei★, Studying for master’s degree, Institute of Orthopedic Diseases, Jinan University, Guangzhou 510630, Guangdong Province, China; Department of Orthopedics, the First Affiliated Hospital of Jinan University, Guangzhou 510630, Guangdong Province, China huansw@sohu.com
  • Supported by:

    The National High Technology Research and Development Program of China (863 Program), No.2007AA09Z440*

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Abstract:

BACKGROUND: Cell transplantation technique for bone tissue engineering is a hotspot in recent years. Scaffold material is an important component, which can obtain the most promising breakthrough among the three elements. With the innovative development of tissue engineering material technology, it is expected to bring a hope for better repair of bone defects.
OBJECTIVE: To investigate the effect of propylene carbonate/chitosan nanofibers (PPC/CSNF) porous scaffolds combined with bone marrow mesenchymal stem cells (BMSCs) to repair bone defects in the femoral condyle of rabbits.
METHODS: PPC/CSNF composite porous scaffold was processed by in situ phase separation, the third passage of BMSCs were cultured on the composite scaffold. Thirty-six New Zealand white rabbits were divided into experimental group, standard group, control group and blank group at random. A bone defect was made, 6 mm in diameter and 10 mm in depth, in the right femoral condyle of each rabbit. Then PPC/CSNF/BMSCs compound was planted into the defected position in the experimental group; autogenous bone in the standard group; PPC/CSNF scaffolds in the control group; and nothing in the blank group. The curative effect was evaluated by general observation, radiographic examination and histologic analysis at the 4th, 8th, and 12th week after operation．
RESULTS AND CONCLUSION: Roentgenographically, the bone defects in the experimental groups and criterion groups exhibited new bone formation increased with time, which were apparently superior to that in the control group (P < 0.05). The quality of new bone formation was significantly different between the experimental and the control groups by histologic analysis. In the blank group, bone defect could not be repaired without proper treatment, finally filling only with fibrous tissue. PPC/CSNF porous scaffold composited with BMSCs has a good ability to accelerate the formation of new bone, and repair bone defect finally.

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