Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (16): 2885-2888.doi: 10.3969/j.issn.1673-8225.2011.16.009

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Application of different bone graft materials in the process of lumbar fusion and transforming growth factor-beta expression

Wang Lei-lei, Jin Ge-le, Ren Long-long, Yang Yi   

  1. Second Department of VIP Surgery, First Affiliated Hospital of Xinjiang Medical University, Urumqi  830000, Xinjiang Uygur Autonomous Region, China
  • Received:2010-10-18 Revised:2011-03-02 Online:2011-04-16 Published:2013-11-11
  • Contact: Jin Ge-le, Doctor, Professor, Chief physician, Second Department of VIP Surgery, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, China jingl81@yahoo.com.cn
  • About author:Wang Lei-lei★, Studying for master’s degree, Second Department of VIP Surgery, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, China 313670898@qq.com
  • Supported by:

    the Natural Science Foundation of Xinjiang Uygur Autonomous Region, No. 2010211a40*; the National Natural Science Foundation of China, No. 30760257*

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Abstract:

BACKGROUND: The effect of bone morphogenetic protein (BMP) used to bone transplantation is not ideal, while transforming growth factor-β (TGF-β) can significantly enhance osteoconductivity in vivo of BMP.
OBJECTIVE: To observe the TGF-β expression on autogenous bone and recombinant human bone morphogenetic protein-2 (rhBMP-2) used for the process of the lumbar fusion of rabbit.
METHODS: Sixty adult male New Zealand white rabbits were randomly divided into autograft group, composite group and allograft group. The rabbits were undergone L5-6 intertransverse process fusion respectively using autograft, rhBMP-2/composite bone and allograft. 3 segmental calluses were obtained at 7, 14, 21, 28, 35 days after implantation, the TGF-β expression was detected by real time fluorescent quantitation RT-PCR.
RESULTS AND CONCLUSION: At 28days after implantation, the TGF-β expression in composite group reached the peak, and then declined, but still had statistical difference compared to the other two groups (P < 0.05). RhBMP-2/composite allograft released rhBMP-2; meanwhile, the rhBMP-2 effectively improved the TGF-β expression and significantly increase osteoinductive effect in vivo.

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