Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (15): 2747-2750.doi: 10.3969/j.issn.1673-8225.2011.15.021

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Inhibitory effect of estrogen replacement therapy on naphthalene-induced oxidative damage of the lens in ovariectomized female rats 

Kang Gang-jin1, 2, Li Ping-hua1   

  1. 1Department of Ophthalmology, the First Affiliated Hospital, Chongqing Medical University, Chongqing  400016, China
    2Department of Ophthalmology, the Affiliated Hospital of Luzhou Medical College, Luzhou  646000, Sichuan Province, China
  • Received:2010-12-06 Revised:2011-03-01 Online:2011-04-09 Published:2013-11-06
  • Contact: Li Ping-hua, Professor, Master’s supervisor, Department of Ophthalmology, the First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China cylipinghua@163. com
  • About author:Kang Gang-jin★, Master, Associate professor, Master’s supervisor, Department of Ophthalmology, the Affiliated Hospital of Luzhou Medical College, Luzhou 646000, Sichuan Province, China kanggangjin2 @sohu.com
  • Supported by:

    Science Foundation of Luzhou Medical College, No. 04113*

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Abstract:

BACKGROUND: The primary research indicated that anti-oxidation may be one of mechanisms of protective effects of estrogen to lens, but the pathway is indefinite, and the effect of clinical methods of estrogen replacement therapy for oxidative damage on lens was reported seldom before.
OBJECTIVE: To investigate the effects of estradiol and estradiol combined with progesterone on lens in opacity, oxidative defense system status, as well as its lipid peroxidative product and soluble protein levels of naphthalene-induced cataract in ovariectomized female rats, also discuss the effects and mechanisms of two clinical methods of estrogen replacement therapy for oxidative damage on lens．
METHODS: Thirty-two adult female Sprague-Dawley rats were divided into four groups randomly: control, model, estradiol, and estradiol combined with progesterone. All rats were prepared for ovariectomized models except that of the control group. After 2 weeks of operation, all rats in each group were received naphthalene for 6 weeks through a stomach tube. The changes of lenses were observed with slit-lamp microscope. After 6 weeks of naphthalene administration, all rats were killed and serum estradiol and progesterone levels were determined with radioimmunoassay in each rat. The rats lenses were taken out and the levels of lens oxidative defense system and soluble protein were examined with biochemical technique.
RESULTS AND CONCLUSION: Compared to the control group, opacity of lens were slight and late in estradiol and estradiol combined with progesterone groups, the level of SOD, GSH, vitamin C and soluble protein were increased (P < 0.05), while the levels of MDA was decreased (P < 0.05). In addition, the level of serum estradiol and progesterone increased significantly (P < 0.01). But there was no significant difference among estradiol, estradiol combined progesterone in all testing items. Both estradiol and estradiol combined with progesterone have inhibitory effect on naphthalene-induced oxidative damage of the lens in ovariectomized female rats, this effect may be performed through promoting antioxidative defense system activity, also reducing lipid peroxidative products, maintaining the level of soluble protein of lens. This inhibitory effect is one of the mechanisms of estrogen protective effects of estrogen replacement therapy on the lens.

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