Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (11): 1933-1936.doi: 10.3969/j.issn.1673-8225.2011.11.008

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Interventional effect of pravastain on core binding factor alpha 1 expression in rabbit models with steroid-induced avascular necrosis of the femoral head

Hu Min1, Fan Jian-nan2, Zhao Hong-bin3, Qian Chuan-yun3, Wei Wei3, Zhang Yang3   

  1. 1Medical College of Kunming University, Kunming  650032, Yunnan Province, China
    2Department of Orthopedics, Affiliated Hospital of Guiyang Medical College, Guiyang  550004, Guizhou Province, China
    3Department of Orthopedics, First Affiliated Hospital of Kunming Medical College, Kunming   650032, Yunnan Province, China
  • Received:2010-11-11 Revised:2010-12-24 Online:2011-03-12 Published:2011-03-12
  • Contact: Zhao Hong-bin, Doctor, Associate professor, Department of Orthopedics, First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan Province, China
  • About author:Hu Min, Associate professor, Medical College of Kunming University, Kunming 650032, Yunnan Province, China humin999@yahoo.cn Fan Jian-nan, Associate professor, Department of Orthopedics, Affiliated Hospital of Guiyang Medical College, Guiyang 550004, Guizhou Province, China Hu Min and Fan Jian-nan contributed equally to this paper.
  • Supported by:

    the National Natural Science Foundation of China, No. 81060361*, 30860389*; Applied Basic Research Fund of Yunnan Province, No. 2010ZC169*; the Foundation of Education Commission of Yunnan Province, No. 2010C212*; the Key Program of Science and Technology Plan of Kunming City, No. 10S090202*

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Abstract:

BACKGROUND: Statins, such as lovastatin and simvastatin, can up regulate expression of core binding factor α1 (cbfα1) and promote repair of osteonecrosis.
OBJECTIVE: To evaluate effect of pravastatin on protein cbfα1 expression in steroid-induced avascular necrosis of the femoral head (SANFH) rabbit models.
METHODS: Totally 80 New Zealand white rabbits were randomly divided into a normal control group (n=18) and experimental group (n=62). Rabbits in the experimental group were prepared for SANFH models. At 5 weeks after model preparation, 36 rabbits in the model group were assigned into the model and pravastatin groups, with 18 animals in each group. Rabbits in the pravastatin group received intragastric administration with 1.2 mg/kg pravastatin once per day, and the same volume of distilled water was given to those in the model and control groups. Rabbits in each group were killed at 8, 12 and 16 weeks respectively to evaluate of protein cbfα1 expression in the femoral heads using immunohistochemistry.
RESULTS AND CONCLUSION: Positive protein expression of cbfα1 could be found in the control group at different time points; which was negative in the model group at 8 and 12 weeks, but weakly positive at 16 weeks. Expression of cbfα1 was positive in the pravastatin groups at 8, 12 and 16 weeks, which gradually enhanced with time prolonged. The results demonstrated that pravastatin can effectively promote protein cbfα1 in early SANFH rabbit models.

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