Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (10): 1741-1744.doi: 10.3969/j.issn.1673-8225.2011.10.007

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Recombinant human bone morphogenetic protein-2 combined with basic fibroblast growth factor promotes vascular endothelial growth factor expression and osteogenic potential of bone marrow stromal cells in rabbits 

Chang Qi1, Huang Chang-lin2, Huang Tao1   

  1. 1Department of Orthopaedics, 2Institute of Military Medicine of PLA, the 150th Central Hospital of Chinese PLA, Luoyang  471031, Henan Province, China
  • Received:2010-09-06 Revised:2010-12-21 Online:2011-03-05 Published:2011-03-05
  • About author:Chang Qi☆, Doctor, Associate chief physician, Department of Orthopaedics, the 150th Central Hospital of Chinese PLA, Luoyang 471031, Henan Province, China changqi1127@yahoo.com.cn
  • Supported by:

    Medical and Health Scientific Research Project of Chinese PLA, No. 06Q024*

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Abstract:

BACKGROUND: Whether recombinant human bone morphogenetic protein-2 (rhBMP-2) and basic fibroblast growth factor (bFGF) can reach the dual role of the strength of osteogenesis and promotion of bFGF expression is need to know in vitro or in vivo experiment.
OBJECTIVE: To investigate the effects of rhBMP-2 and bFGF on the vascular endothelial growth factor (VEGF) expression and osteogenic potential of marrow stromal cells (MSCs).
METHODS: The MSCs of the femur of the rabbit were procured and cultured by MSCs cultured in vitro. The samples were treated with rhBMP-2 or (and) bFGF. After culturing for five days, the morphology of cells, proliferation, alkaline phosphatase activities (APA), bone clusters, and the ratio of VEGF-positive cells were detected.
RESULTS AND CONCLUSION: The effects of combined use of BMP-2 and bFGF on cell counting, APA, percentage of mineralized area, and the ratio of VEGF-positive cells were better than that of using rhBMP-2 or bFGF singly. The results showed that a rational combined use of rhBMP-2 and bFGF can not only enhances multiplication ability and osteogenic transformation of MSCs, but also promotes VEGF expression of the proliferation of VEGF.

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