Abstract:

BACKGROUND: Due to serious myelosuppression caused by hemopoietic stem cell damage when using cisplatin chemo-treatment, there is no effective cytoprotective agent of chemo-treatment to lessen its adverse reaction.
OBJECTIVE: To explore the protection of hydrogen sulfide (H2S) against cisplatin–induced human bone marrow mesesenchymal stem cells (hBMSCs) damage.
METHODS: Sodium hydrosulfide (NaHS) as a H2S donor. hBMSCs treated with different concentrations of sodium hydrosulfide and cisplatin for 48 hours, the cell survival rate was measured by MTT assay; the morphological change of apoptotic cells was tested by using the chromatin dye Hoechst 33258; the proteins expression were detected with Western blot techniques.
RESULTS AND CONCLUSION: MTT detection showed that cisplatin chould reduce the survival rate of BMSCs, and NaHS dose-dependently blocked the inhibition of hBMSCs cells growth induced by cisplatin. When hBMSCs cells were treated by both NaHS (0.5, 1 mmol/L) and cisplatin (20 mg/L) for 48 hours, the NF-κB (p65) was up-regulated, but the IκB-α was down-regulated. When hBMSCs cells were treated by both NaHS (1 mmol/L) and cisplatin (20 mg/L) for 6 hours, the level of phosphorylated protein kinase 1/2 was significantly ascended. The results showed that H2S chould protect hBMSCs against cisplatin-induced damage, which may be associated with the activation of protein kinase-NF-κB signaling pathway.