Mechanical properties of rat tibia under high-G environment

doi:10.3969/j.issn.2095-4344.2485

Abstract

Abstract:

BACKGROUND: With the development of Chinese space industry, pilots are exposed to high-G mechanical environment, which will seriously affect their bones. However, as one of the bones that are most prone to fracture, the biomechanical behavior of the tibia under extreme mechanical environment is rarely reported.

OBJECTIVE: To explore the effects of different high-G mechanical environments on the rat growth and development and the mechanical properties of the tibia.

METHODS: Male Wistar rats were provided by Laboratory Animal Center of Academy of Military Sciences. The cantilever was set to run at different speeds and accelerations with a high-G centrifugal loading device, and the high-G rat model was made. The rats were weighed weekly. The left tibia of rats was used for undergoing a three-point bending experiment, and the deflection and elastic modulus of the tibia were calculated. The right tibia was subjected to a creep test, and constant stress was applied on the surface of tibial cortical bone and kept 3 600 seconds, so as to observe the changes of creep strain. The study was approved by the Laboratory Animal Ethical Committee of Tianjin University of Technology.

RESULTS AND CONCLUSION: The high-G environment affected the normal growth and development of rats, inhibited weight gain and reduced the mechanical properties of the tibia, and reduced the limit deflection of the tibia by 8.1%, 12.2%, 37.8%, and 51.4%, respectively. Limit loads were decreased by 16%, 9%, 25.2%, and 29% respectively. To conclude, extremely high-G environment exerts serious negative effects on rats.

Key words: high-G, tibia, weight, mechanical properties, three-point bending, cortical bone, biomechanics, tissue engineering

CLC Number:

Liu Jin, Gao Lilan, Peng Yulin, Zhang Xizheng. Mechanical properties of rat tibia under high-G environment[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(11): 1654-1658.

Figures/Tables 5

References

[1] 钟国徽,李玉恒,凌树宽,等.太空微重力环境对人体的影响及防护措施[J].生物学通报,2016,51(10):1-4.
[2] SPECTOR ER, SMITH SM, SIBONGA JD. Skeletal effects of long-duration head-down bed rest. Aviat Space Environ Med. 2009;80(5 Suppl):A23-28.
[3] VICO L, BAROU O, LAROCHE N, et al. Effects of centrifuging at 2 g on rat long bone metaphyses. Eur J Appl Physiol Occup Physiol. 1999;80(4):360-366.
[4] IKAWA T, KAWAGUCHI A, OKABE T, et al. Hypergravity suppresses bone resorption in ovariectomized rats. Advances in Space Research.2011;47(7):1214-1224.
[5] AMTMANN E, OYAMA J. Effect of chronic centrifugation on the structural development of the musculoskeletal system of the rat. Anat Embryol (Berl). 1976;149(1):47.
[6] 林俊豪.不同加速度的高G极端环境对骨组织影响的生物力学实验研究[D].长春:吉林大学,2018.
[7] 陈孟诗,赖胜祥,李良,等.大鼠的骨生物力学指标选取及测试[J].生物医学工程学杂志,2001,18(4):547.
[8] 陈天华,陈孟诗,姚晓琳,等.一种新的测试胫骨骨生物力学特性的三点弯曲方法[J].生物医学工程学杂志, 2008,25(2):341-343.
[9] 蒋超,王以朋,邱贵兴,等. 晚期骨关节炎患者膝关节软骨下松质骨祖细胞的特性研究[J].中国骨与关节外科,2016,9(1):69-74.
[10] SASAKI N, NAKAYAMA Y, YOSHIMAWA M, et al. Stress relaxation function of bone and bone Collagen. Biomech.1993;26: 1369-1376.
[11] 张伊卓,宋雅伟,卞雯文.股骨颈的实验力学研究[J].中国骨质疏松杂志,2015,21(9):1065-1068.
[12] IYO T, MAKI Y, SASAKI N, et al. Anisotropic viscoelastic properties of cortical bone. Biomech.2004; 37:1433-1437.
[13] 吴丹凯,李新颖,李鹏.老年胸腰椎松质骨的扭转力学特性[J].中国老年学,2015,35(1):180-181.
[14] JI B, GAO H. Mechanical properties of nanostructure of biological materials. Mech Phys Solids.2004;52:1963-1990.
[15] 张翘,张淑娟,李新颖.青年与老年胸椎松质骨应力松弛特性对比分析[J].中国老年学,2015,35(2): 395-396.
[16] 魏朝磊,高丽兰,张春秋,等.单轴压缩下松质骨的应力率和蠕变性能[J].中国组织工程研究,2017,21(31): 5014-5018.
[17] 郭长青,张丽萍.针刀干预对膝骨关节炎兔髌韧带拉伸、蠕变及应力松弛等生物力学特性的影响[J].中国科学:生命科学, 2016,46(8): 976-982.
[18] 魏朝磊.过载条件下松质骨力学性能的研究[D].天津:天津理工大学, 2018.
[19] 杨洁.福尔马林防腐对牛股骨皮质骨生物力学性能的影响研究[D].长沙:湖南大学,2016.
[20] ALBERT CI, JAMESON J, HARRIS G. Design and validation of bending test method for characterization of miniature pediatric cortical bone specimens. Proc Inst Mech Eng H. 2013;227(2): 105-113.
[21] 高子剑,于露,高丽兰,等.大鼠高加速度离心加载装置:高加速度环境下动物的力学生物学响应[J].中国组织工程究, 2019,23(3):372-377.
[22] 曹新生,吴兴裕,孙喜庆,等.低G预适应对高G暴露致大鼠学习记忆功能受损的影响[J].南方医科大学学报,2005,25(2):212-215.
[23] 赵波,吕云利,孙喜庆.高性能战机加速度致脑缺血预警与防护研究进展[J].西北国防医学杂志,2017,39(6): 415-417.
[24] 陈铭,关键,段峰,等.机械离心力对成骨细胞骨形态发生蛋白信号通路的影响[J].中国组织工程研究, 2012,16(20):362-3628.
[25] 张舒.不同重力环境对大鼠成骨细胞力学信号转导功能的影响[D].西安:第四军医大学,2000.
[26] 丁柏,汪恭质,张晓铀,等.模拟失重和超重刺激对体外培养成骨瘤细胞分裂,增殖影响的研究[J].航天医学与医学工程, 1997,10(2): 104-107.
[27] MiWA M, KOZAWA O, TOKUDA H, et al. 超重力对成骨细胞样细胞增殖和分化的影响[J].骨性矿工, 1991,14(1):15-25.