Guishen Pill water extract intervenes with
the proliferation of bone marrow mesenchymal stem cells and expression levels
of PI3K and AKT in Sprague-Dawley rats

doi:10.3969/j.issn.2095-4344.2051

Abstract

Abstract:

BACKGROUND: The effect of kidney-tonifying prescriptions on proliferation of bone marrow mesenchymal stem cells has been verified, but its mechanism of action is still unclear. In this study, we took Guishen Pil as an example to explore its functional mechanism.

OBJECTIVE: To explore the relationship between Guishen Pill nourishing kidney essence and proliferation of bone marrow mesenchymal stem cells by observing the effect of Guishen Pill water extract on the proliferation of bone marrow mesenchymal stem cells.

METHODS: Bone marrow mesenchymal stem cells were isolated from the femoral bone marrow of 3-week-old male Sprague-Dawley rats and cultured in vitro. The cells were purified using the whole bone marrow adherence method, and the passage 3 cells of higher purity were selected for the drug intervention. The cells were divided into blank control group and 10, 20, 40, 80 and 100 mg/L Guishen Pill groups. The dose-effect and time-effect relationships were investigated after cultured for 5 days. The cell morphology was observed under inverted microscope. The cell proliferation was evaluated by cell counting kit-8 assay. The expression levels of PI3K and AKT proteins were detected by western blot assay.

RESULTS AND CONCLUSION: Guishen Pill significantly increased the cell proliferation in the Guishen Pill groups in a dose- and time-effect manner. The proliferation of bone marrow mesenchymal stem cells in the 80 mg/L Guishen Pill group was strongest at the 3^rd day. The expression levels of PI3K and AKT proteins in the Guishen Pill groups were significantly higher than those in the blank control group. In summary, Guishen Pill water extract can effectively boost the proliferation of bone marrow mesenchymal stem cells, which may be related to its involvement in regulating PI3K/AKT signaling pathway.

Key words: bone marrow mesenchymal stem cells, Guishen Pill, cell proliferation, PI3K, AKT, kidney essence

CLC Number:

Liu Yan, Guan Yongge, Song Yang. Guishen Pill water extract intervenes with the proliferation of bone marrow mesenchymal stem cells and expression levels of PI3K and AKT in Sprague-Dawley rats[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(13): 1983-1988.

Figures/Tables 6

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