Activity of intervertebral disc stem cells and nucleus
pulposus mesenchymal stem cells in intervertebral disc niche condition

doi:10.3969/j.issn.2095-4344.2041

Abstract

Abstract:

BACKGROUND: Disc microenvironment plays an important role in the biological behavior of stem cells. It is proposed that the microenvironment can be used to achieve the repair of disc tissue independent of seed cells by niche regulation.

OBJECTIVE: To explore the difference in the activity of intervertebral disc stem cells and nucleus pulposus mesenchymal stem cells under the intervertebral disc niche.

METHODS: Ten healthy male Sprague-Dawley rats were used. The intervertebral disc bone tissue was isolated according to the anatomical area. Intervertebral disc stem cells were digested with type II collagenase and cultured in vitro. Nucleus pulposus was isolated, and nucleus pulposus mesenchymal stem cells were cultured in vitro using enzyme digestion. Intervertebral disc stem cells and nucleus pulposus mesenchymal stem cells were cultured and amplified in normal condition and intervertebral disc niche. The proliferation curves of cultured cells were measured by MTT method at 1-6 days after culture. The expression level of CD29 was detected by flow cytometry at 1, 3 and 6 days.

RESULTS AND CONCLUSION: Under different culture conditions, the proliferation of intervertebral disc stem cells and nucleus pulposus mesenchymal stem cells showed an opposite trend. Under normal culture conditions, intervertebral disc stem cells and nucleus pulposus mesenchymal stem cells were proliferated logarithmically from day 4 to day 6, with no statistical significance (P > 0.05). In the intervertebral disc niche condition, the survival rate of nucleus pulposus mesenchymal stem cells was significantly lower than that of intervertebral disc stem cells (P < 0.05). In intervertebral disc niche condition, at 3 and 6 days, the surface positive antigen CD29 of intervertebral disc stem cells was significantly higher than that of nucleus pulposus mesenchymal stem cells (P < 0.05). These results suggest that in the intervertebral disc niche condition, the activity of intervertebral disc stem cells and nucleus pulposus mesenchymal stem cells is inhibited to some extent, but intervertebral disc stem cells are more active than nucleus pulposus mesenchymal stem cells.

Key words: intervertebral disc, intervertebral disc stem cells, nucleus pulposus mesenchymal stem cells, CD29, cell proliferation

CLC Number:

Hu Wei, Tong Min, Yang Tao, Xiong Wei, Huang Yifei. Activity of intervertebral disc stem cells and nucleus pulposus mesenchymal stem cells in intervertebral disc niche condition [J]. Chinese Journal of Tissue Engineering Research, 2020, 24(19): 2953-2958.

Figures/Tables 3

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