Chinese Journal of Tissue Engineering Research ›› 2017, Vol. 21 ›› Issue (13): 2009-2014.doi: 10.3969/j.issn.2095-4344.2017.13.008

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Effects of recombinant adenovirus vector expressing human lactoferrin on proliferation and apoptosis of cervical cancer stem-like cells

Zhang Xia, Du Zhi-xiang, Wang Na, Meng Ya-li   

  1. First Hospital of Hebei Medical University, Shijiazhuang 050031, Hebei Province, China
  • Revised:2017-03-22 Online:2017-05-08 Published:2017-06-09
  • Contact: Meng Ya-li, Chief physician, the First Hospital of Hebei Medical University, Shijiazhuang 050031, Hebei Province, China
  • About author:Zhang Xia, Master, Attending physician, the First Hospital of Hebei Medical University, Shijiazhuang 050031, Hebei Province, China
  • Supported by:

    the Key Project of Medical Science Research in Hebei Province in 2014, No. ZD20140325

Abstract:

BACKGROUND: Gene engineering plays an important role in the process of human malignant tumor treatment, and adenovirus vectors for gene therapy are commonly used. In recent years, lactoferrin is found to exert important effects on the occurrence, development and metastasis of a variety of tumors. However, little is reported on the in vivo expression of adenovirus vector mediated lactoferrin in the human body.
OBJECTIVE: To investigate the effect of adenovirus mediated human lactoferrin (Ad-hLF) on proliferation and apoptosis of cervical cancer stem-like cells.
METHODS: Primary cervical cancer cells from mice were cultured in vitro to sort cervical cancer stem-like cells using SP method. Afterwards, the stem-like cells were divided into three groups, blank control group, empty vector group, and Ad-hLF group, followed by transfection with nothing, Ad-GFP and Ad-hLF, respectively. After transfection, MTT assay was used to detect the proliferation of cervical cancer stem-like cells; scratch-wound assay was employed to detect the migration of cervical cancer stem-like cells; western blot assay was used to determine the expression of lactoferrin in cervical cancer stem-like cells; and Annexin V-FITC/PI was used to detect cell apoptosis.
RESULTS AND CONCLUSION: Compared with the blank control and empty vector groups, Ad-hLF significantly inhibited the proliferation of cervical cancer stem-like cells and reduced the number of scratched cells, while the cell apoptosis increased in the Ad-hLF group (P < 0.05 or P < 0.01). These findings indicate that the recombinant adenovirus vector mediated lactoferrin Ad-hLF for transfection of cervical cancer stem-like cells can be stably expressed in cervical cancer stem-like cells, and can inhibit the proliferation, increase apoptosis and reduce migration of cervical cancer stem-like cells.

 

中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程

Key words:  Stem Cells, Tissue Engineering, Cell Proliferation

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