Effects of exogenous stem cell factors on the gastric electrical activity and impedance in a mouse model of diabetic gastroparesis

doi:10.3969/j.issn.2095-4344.2017.01.023

Abstract

Abstract:

BACKGROUND: Lesions in Cajal interstitial cells can lead to diabetic gastroparesis, in which, insulin and stem cell factor are likely to act as upstream regulatory factors.
OBJECTIVE: To investigate the effect of exogenous stem cell factors on the gastric electrical activity and gastric impedance in a mouse model of diabetic gastroparesis.
METHODS: Forty Wistar rats were randomly divided into two groups, 20 rats in each group. The diabetic gastroparesis model was prepared in observation group by a single use of 60 mg/kg streptozotocin via intraperitoneal injection and high-sugar/high-fat diet. Rats in control group were given the equal volume of normal
saline and normal diet. Eight weeks later, residual rate of gastric pigment was detected, and gastric antrum microstructures were observed under transmission electron microscope. If the animal model was successfully prepared, rats were intraperitoneally given 0.4 μg/kg/d stem cell factor in the observation group and same volume of PBS in the control group, for consecutive 15 days. Afterwards, the impedance gastric motility system was detected.
RESULTS AND CONCLUSION: Compared with the control group, the residual rate of gastric pigment was significantly higher in the observation group (P < 0.05). In the observation group, there was a certain change in the Cajal interstitial cells in the antrum of stomach, such as the gap junction between the cells were significantly reduced and became loose; there was a lack of continuity of the basal lamina; the number of organelles in the cells was significantly decreased, the cytoplasm was dissolved, and a large number of vacuoles appeared. After treatment with exogenous stem cell factor, the gastric electrical activity and gastric impedance of rats were significantly improved: for the gastric electrical activity, the percentage of < 2 band power was significantly decreased, and the percentage of 2-4 band power increased significantly (P < 0.05); for the gastric impedance, the percentage of < 2 band power was significantly decreased, and the percentage of 2-4 band power increased significantly (P < 0.05). After treatment, no significant difference in the gastric electrical activity and gastric impedance was found between the two groups. The experimental results show that delayed gastric emptying may occur in diabetic gastroparesis rats, and the degeneration of Cajal interstitial cells may induce gastroparesis. Exogenous stem cells can effectively improve the level of gastric electrical activity and gastric impedance, and thereby improve the gastric motility in rats.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stem Cell Factor, Interstitial Cells of Cajal, Diabetes Mellitus, Gastroparesis, Tissue Engineering

CLC Number:

R394.2

Gao Jin-liang, Zhao Nan, Song Zhan. Effects of exogenous stem cell factors on the gastric electrical activity and impedance in a mouse model of diabetic gastroparesis[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(1): 128-132.

Figures/Tables 3

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