Correlation between growth, proliferation and apoptosis of leukemia cell lines K562/AO2 and bone marrow mesenchymal stem cells in children with leukemia

doi:10.3969/j.issn.2095-4344.2016.41.005

Abstract

Abstract:

BACKGROUND: Leukemia comes from the damage to bone marrow microenvironment, and changes in bone marrow microenvironment lead to some effects on biological characteristics and behavior of leukemia cells.
OBJECTIVE: To explore the effect of bone marrow mesenchymal stem cells from children with leukemia on K562/AO2 cell line growth, proliferation and apoptosis.
METHODS: K562/AO2 cells were cultured alone in vitro, or co-cultured with bone marrow mesenchymal stem cells from leukemia children. The living cells in the two groups were counted using trypan blue staining, and the cell growth curves were described. PI single staining method was used to detect the cell cycle, and cell apoptosis was detected by V/PI Annexin method in the two groups.
RESULTS AND CONCLUSION: After single suspension culture, the cells showed no marked changes within the first 3 days, but began to proliferate fast at 4 days and reached the proliferation peak at 6 days. Co-cultured cells showed a gentle growth curve with no presence of obvious proliferation peak. Compared with the co-culture group, the proportion of G₀/G₁ phase cells was significantly lower, but the proportion of S phase cells was significantly higher in the single culture group (P < 0.05). The number of cells in the G2/M phase showed no significant difference between the two groups (P > 0.05). All these findings indicate that co-culture with bone marrow mesenchymal stem cells from leukemia children inhibits the proliferation of K562/AO2 cells through G₀/G₁ phase arrest, and also resists apoptosis in K562/AO2 cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Leukemia, Bone Marrow, Mesenchymal Stem Cells, Cell Proliferation, Apoptosis, Tissue Engineering

CLC Number:

R394.2

Lei Chun-xia. Correlation between growth, proliferation and apoptosis of leukemia cell lines K562/AO2 and bone marrow mesenchymal stem cells in children with leukemia[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(41): 6112-6117.

Figures/Tables 2

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