Icariin promotes osteogenic differentiation of bone marrow mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2016.24.001

Abstract

Abstract:

BACKGROUND: Herba epimedii, a traditional Chinese medicine, has a long time in dealing with various orthopedic disorders. Icariin with many biological activites is one of the most important compositions of Herba epimedii.
OBJECTIVE: To investigate the effects of icariin on osteogenic differentiation of mesenchymal stem cells and the underlying mechanisms.
METHODS: Bone marrow mesenchymal stem cells were treated using icariin with or without osteogenic medium in vitro. Osteogenic differentiation markers, including runt-related transcription factor 2, osteocalcin and osterix, were detected by real time-qPCR. Alizarin red staining was used to measure calcium nodes generated by osteoblasts induced from bone marrow mesenchymal stem cells. The proximal tibia bone structure of rats fed with icariin (2 mg per day) for 5 weeks was detected and analyzed by MicroCT.
RESULTS AND CONCLUSION: Icariin was able to promote the expression of genes related to osteogenic differentiation in the absence or presence of osteogenic induction. Icariin could obviously increase the quantity of calcium nodes when mesenchymal stem cells were cultured in the osteogenic medium. The animal experiment showed that icariin improved formation of trabecular bone.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Epimedium, Bone Marrow, Mesenchymal Stem Cells, Osteocalcin, Core Binding Factor Alpha 1 Subunit, Tissue Engineering

CLC Number:

R318

Bao Yuan, Huang Jun-ming, Jing Xing-zhi, Li Xing-yan, Dong Yong-hui, Zhang Jin-ming, Guo Feng-jin, Chen An-min. Icariin promotes osteogenic differentiation of bone marrow mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(24): 3501-3507.

Figures/Tables 2

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