Feasibility of umbilical cord blood stem cell transplantation for the treatment of diabetic lower limb ischemia

doi:10.3969/j.issn.2095-4344.2016.01.014

Abstract

Abstract:

BACKGROUND: Diabetic lower limb ischemia is prone to involve distal lower limb arteries, and a conventional treatment is often unable to obtain the ideal effect.
OBJECTIVE: To investigate the effect and safety of umbilical cord blood stem cell transplantation in the treatment of diabetic lower limb ischemia.
METHODS: A diabetic rat model of lower limb ischemia was established, and along the femoral artery, five points
were selected for injection of human umbilical cord mesenchymal stem cell suspension, 20 μL per point. At 1, 2, 4 weeks after transplantation, transcutaneous oxygen pressure, vascular density and vascular endothelial growth factor level in the ischemic region, and incidence of adverse reactions were recorded.
RESULTS AND CONCLUSION: At 1, 2 and 4 weeks after transplantation, the transcutaneous oxygen pressure, vascular density and vascular endothelial growth factor level in the ischemic region were found increasing, which were significantly different from those before transplantation (P < 0.05). At different time after transplantation, all animals had no inflammatory reactions such as skin bleeding and dermatitis, and local red, swelling, hot, pain, and had no tumor-like growth in organs. These findings indicate that umbilical cord blood stem cell transplantation can safely and significantly improve symptoms of diabetic lower limb ischemia, which has certain application feasibility.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

CLC Number:

R394.2

Xie Li-hua, Xing Lin, Zheng Hang. Feasibility of umbilical cord blood stem cell transplantation for the treatment of diabetic lower limb ischemia[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(1): 78-82.

Figures/Tables 5

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