Culture, differentiation, proliferation and invasion of tumor stem cells in human esophageal carcinoma cell lines KYSE-150 and TE-1

doi:10.3969/j.issn.2095-4344.2016.01.010

Abstract

Abstract:

BACKGROUND: There is a certain cell subset in esophageal cancer tissues, with certain invasive and metastatic properties, which is closely related to the clinical therapeutic effect on tumors.
OBJECTIVE: To isolate tumor stem cell spheres in human esophageal carcinoma cell lines KYSE-150 and TE-1 and to analyze their proliferation and invasion ability.
METHODS: KYSE-150 and TE-1 cells were cultured in serum-free medium to observe the formation of cell spheres. Cell proliferation and invasion were detected using MTT and Transwell chamber culture. Surface markers of cells were detected using flow cytometry.
RESULTS AND CONCLUSION: Cell spheres that were stably subcultured were obtained from KYSE-150 and TE-1 cells cultured in serum-free medium. The proliferation and invasion abilities of cell spheres were significantly stronger than those of parent cells (P < 0.05). The number of CD44+, CD271+ and CD44+CD271+ cells in TE-1 and KYSE-150 cell spheres was significantly higher than that in the TE-1 and KYSE-150 parent cells (P < 0.05). These experimental results show that cell spheres isolated from human esophageal carcinoma cell lines TE-1 and KYSE-150 have tumor stem cell properties as well as strong proliferation and invasion abilities. And moreover, CD44 and CD271 can be used as important surface markers of esophageal carcinoma stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

CLC Number:

R394.2

Wang Yong-lian, Wang Zhong-min, Wang Yi, Tao Yi-peng, Han Gao-yang . Culture, differentiation, proliferation and invasion of tumor stem cells in human esophageal carcinoma cell lines KYSE-150 and TE-1[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(1): 55-59.

Figures/Tables 4

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