Dimethyl sulfoxide induces differentiation of bone marrow mesenchymal stem cells into myocardial cells

doi:10.3969/j.issn.2095-4344.2016.01.005

Abstract

Abstract:

BACKGROUND: Numerous studies have demonstrated that bone marrow mesenchymal stem cells can be induced to differentiate into myocardial cells under certain conditions. Dimethyl sulfoxide is one of the commonly used inducers, and its mechanism is mainly by inhibiting the c-myc gene expression, thus reducing endogenous poly(adenosine diphosphate nucleotide) level.

OBJECTIVE: To study the feasibility of dimethyl sulfoxide inducing the myocardial differentiation of bone marrow mesenchymal stem cells and its optimal concentration.

METHODS: Bone marrow mesenchymal stem cells from Sprague-Dawley rats were isolated and cultured in vitro, and then induced by dimethyl sulfoxide to differentiate into myocardial cells. According to the concentrations of dimethyl sulfoxide, there were three groups: 0.6%, 0.8% and 1.0% group. Additionally, a blank control group with no induction was set up. After 72 hours of induction, induction media were removed, and cells were then cultured in normal media for 4 weeks.

RESULTS AND CONCLUSION: Morphology and immunocytochemistry detection results confirmed that dimethyl sulfoxide could induce the differentiation of bone marrow mesenchymal stem cells into myocardial cells in vitro, and differentiated cells expressed desmin, α-actin, cTnT, cTnI and P38MAPK. The optimal induced concentration of dimethyl sulfoxide was 1.0%. Immunofluorescence double staining and electron microscope results further confirmed that dimethyl sulfoxide could induce the myocardial differentiation of bone marrow mesenchymal stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

CLC Number:

R394.2

Sun Long-yun. Dimethyl sulfoxide induces differentiation of bone marrow mesenchymal stem cells into myocardial cells [J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2016.01.005.

Figures/Tables 5

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