Migration and localization of umbilical cord mesenchymal stem cells implanted into brain injury model rats

doi:10.3969/j.issn.2095-4344.2016.01.006

Abstract

Abstract:

BACKGROUND: Choosing an effective means to label and trace the distribution, differentiation and migration of cells in vivo help to further explore the specific mechanism of cells that exert a therapeutic effect.

OBJECTIVE: To understand the migration and localization of BrdU-labeled human umbilical cord mesenchymal stem cells in brain injury model rats.

METHODS: Human umbilical cord blood samples were obtained, and the isolation of human umbilical cord mesenchymal stem cells was carried out. The primary and passage culture were performed. The phenotype of cells was detected by flow cytometry. Passage 3 human umbilical cord mesenchymal stem cells were labeled using BrdU, and the cell proliferation was detected using MTT method. BrdU-labeled cells were injected into brain injury rats via the tail vein. At 14 days after transplantation, brain tissues in the injury region were cut into sections and the migration and location of the umbilical cord mesenchymal stem cells were observed under inverted fluorescence microscope.

RESULTS AND CONCLUSION: Cell surface specific markers CD45 and CD34 were detected by flow cytometry, but the cells could not express CD44, CD105 and CD29. Based on the cell growth curve, the cells came into a conditioning period at 1-3 days of seeding and came into a logarithmic phase at 3-5 days. BrdU-positive cells were visible at the injury region after 14 days, indicating that in the rats, transplanted human umbilical cord mesenchymal stem cells migrated from the peripheral blood to the site of brain injury to achieve the effective repair of injured parts.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

CLC Number:

R394.2

Liu Hong-lin, Liu Zhi-jun, Chen Xiao-bing, Hu Wen-zhong, Ding Bing-qian. Migration and localization of umbilical cord mesenchymal stem cells implanted into brain injury model rats[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(1): 31-35.

Figures/Tables 4

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