Modified cell perfusion appliance for improving the separation efficacy of cardiomyocytes

doi:10.3969/j.issn.2095-4344.2015.51.018

Abstract

Abstract:

BACKGROUND: Separating mature cardiomyocytes is is a complex process. Until now, there is no uniform, high-yield, simple method to harvest cardiomyocytes with high viability.
OBJECTIVE: To explore a more effective method of isolating adult rat cardiomyocytes by modifying the cell perfusion appliance.
METHODS: Cardiomyocytes from adult Sprague-Dawley rats were isolated by collagenase type II perfusion method via a cell perfusion appliance. Traditional Langendorff cell perfusion appliance was used in control group, and modified Langendorff cell perfusion appliance used in experimental group. Under an optical microscopy, morphology of cardiomyocytes was observed and rod-shaped cell ratio was detected to calculate the yield of viable cells. Cardiomyocyte viability was evaluated by trypan blue exclusion test.
RESULTS AND CONCLUSION: The yield of viable cells per rat was significantly higher in the experimental group than in the control group [(3.7±0.5)×107 vs. (2.1±0.4)×107, P < 0.05]. The ratio of trypan blue test-negative cells was also significantly higher in the experimental group than in the control group [(85.6±5.5)% vs. (71.8±5.7)%, P < 0.05]. These findings indicate that high-yield and high-viability cardiomyocytes can be harvested
using the modified Langendorff cell perfusion appliance, which improves the separation efficiency of mature cardiomyocytes and lays the foundation for mature cardiomyocyte culture.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

CLC Number:

R318

Zhang Lin, Zong Jing, Zhang Qing-tao. Modified cell perfusion appliance for improving the separation efficacy of cardiomyocytes[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(51): 8300-8304.

Figures/Tables 2

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