Association between hypoxia inducible factor-1alpha and apoptosis-related proteins in the myocardial tissues under hypoxic training

doi:10.3969/j.issn.2095-4344.2015.51.019

Abstract

Abstract:

BACKGROUND: There are many comparative studies addressing the effect of exercise on apoptosis of cardiomyocytes, but few of them are reported on myocardial apoptosis after hypoxic training. It is unclear whether hypoxia-inducible factor 1α has some effects on cell apoptosis through controlling the expression of Bcl-2 family.
OBJECTIVE: To investigate the relationship between the hypoxia-inducible factor 1α and apoptosis-related protein in the cardiomyocytes during hypoxic training.
METHODS: Sixty male healthy Sprague-Dawley rats were randomly divided into six groups: feeding at room temperature under normoxia and no training (control group); feeding at room temperature under hypoxia and no training (hypoxia group); normoxic training at room temperature (training group); feeding under normoxia+hypoxic training alternating with normoxic training; feeding under hypoxia and normoxic training; feeding under hypoxia+hypoxic training alternating with normoxic training. Rats in the exercise group were subjected to
treadmill training under loading for 8 weeks, 6 days a week. The moving speed and time were increased from 10 m/min and 15 minutes to 25 m/min and 50 minutes at the 8th week, respectively. Hypoxia degree was increased from 1 500 meters above sea level to 3 600 meters above sea leave at the 8th week. After training, hematoxylin-eosin staining, TUNEL method and western blot assay were used to detect the expressions of hypoxia-inducible factor 1α, Bcl-2 and Bax in the myocardial tissues of rats.
RESULTS AND CONCLUSION: Under normoxia, hypoxia-inducible factor 1α rarely expressed; while the expression of hypoxia-inducible factor 1α increased under hypoxia, especially under hypoxic training. Compared with the control group, Bax expression in the myocardial tissues had no changes in the hypoxia group and hypoxic training group, but Bcl-2 expression in these two groups was significantly lowered. Apoptosis in the myocardial tissues was obvious in the hypoxia, training and hypoxic training groups, but no significant difference was found among the three groups. These findings indicate that hypoxic training is unable to increase cell apoptosis and hypoxia-inducible factor 1α can control the cell apoptosis by regulating Bcl-2 and Bax expression.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

CLC Number:

R318

Zou Zhi-bing. Association between hypoxia inducible factor-1alpha and apoptosis-related proteins in the myocardial tissues under hypoxic training [J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2015.51.019.

Figures/Tables 5

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