Construction of diabetic rat models: protective effect of Klotho gene transfer on coronary artery

doi:10.3969/j.issn.2095-4344.2015.49.015

Abstract

Abstract:

BACKGROUND: Klotho gene has an important correlation with organism aging, metabolism and disease. It is confirmed by mouse experiments that Klotho gene can slow down and inhibit atherosclerosis, and its underlying mechanism is involved in lipid metabolism.
OBJECTIVE: To establish diabetic rat models and observe whether Klotho gene transfer has a protective effect on coronary artery of diabetic rats.
METHODS: Klotho gene was extracted from the kidney tissue of normal Sprague-Dawley rats. The target gene was amplified by polymerase chain reaction. Adenovirus was taken as a carrier. Sprague-Dawley rats were randomly divided into model, control and treatment groups to establish diabetic rat models. Klotho gene was transferred into the treatment group, and common adenovirus was transferred into the control group. Rats in model group did not receive any treatment. At the 12th week after modeling, the model animals were sacrificed. Serum low density lipoprotein, high density lipoprotein concentrations, coronary artery intima-media thickness ratio were determined.
RESULTS AND CONCLUSION: The high density lipoprotein concentration in treatment group was significantly higher than that in the model and control groups (P < 0.05). There was no significant difference in high density lipoprotein concentration between model and control groups. The low density lipoprotein in each group was significantly increased, but the differences were not significant (P > 0.05). The intima thickness in treatment group
was significantly smaller than that in model and control groups (P < 0.01), but the differences between model and control groups were not significant (P > 0.05). There was no significant difference in media thickness between each group (P > 0.05). The intima-media thickness ratio in the treatment group was significantly lower than that in the model and control groups (P < 0.01). There was no significant difference in the intima-media thickness ratio between treatment and normal groups (P > 0.05). These results showed that after Klotho gene transfer, the intima-media thickness ratio was substantially close to the normal level, and lower than that in the the model and control groups, and the degree of intimal thickening reduced. These results suggest that Klotho gene has a protective effect on coronary artery after being transferred into diabetic rats.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

CLC Number:

R318

Shu Li-liang, Chen Mao-lin, Su Gang, Xu Jing. Construction of diabetic rat models: protective effect of Klotho gene transfer on coronary artery[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(49): 7958-7963.

Figures/Tables 6

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