Establishing an animal model of ischemic stroke with photothrombosis method

doi:10.3969/j.issn.2095-4344.2015.49.014

Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (49): 7951-7957.doi: 10.3969/j.issn.2095-4344.2015.49.014

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Establishing an animal model of ischemic stroke with photothrombosis method

Ma Jun-ning^{1, 2}, Gao Jun-wei^{1, 2}, Hou Bo-ru¹, Ren Hai-jun^{1, 2}, Liu Ji-xing¹, Chen Si-hua¹, Yan Gui-zhong¹

¹Department of Neurosurgery, Lanzhou University Second Hospital, Lanzhou 730000, Gansu Province, China; ²Lanzhou University School of Life Sciences, Lanzhou 730000, Gansu Province, China

Received:2015-09-03 Online:2015-11-30 Published:2015-11-30
Contact: Ren Hai-jun, Professor, Master’s supervisor, Chief physician, Department of Neurosurgery, Lanzhou University Second Hospital, Lanzhou 730000, Gansu Province, China; Lanzhou University School of Life Sciences, Lanzhou 730000, Gansu Province, China. Corresponding author: Gao Jun-wei, M.D., Associate chief physician, Department of Neurosurgery, Lanzhou University Second Hospital, Lanzhou 730000, Gansu Province, China; Lanzhou University School of Life Sciences, Lanzhou 730000, Gansu Province, China
About author:Ma Jun-ning, Studying for master’s degree, Department of Neurosurgery, Lanzhou University Second Hospital, Lanzhou 730000, Gansu Province, China; Lanzhou University School of Life Sciences, Lanzhou 730000, Gansu Province, China
Supported by:
the Natural Science Foundation of Gansu Province, No. 145RJZA146, 1208RJZA184

Abstract

Abstract:

BACKGROUND: Animal model of experimental cerebral ischemia stroke is an indispensable tool in the research of stroke pathology and the development of novel treatment. The animal model of cerebral ischemia stroke established with the photothrombosis method has simple operation, good stability and high repeatability, it can control the position and size of infarction loci, and therefore it is the preferred choice for studying the pathological mechanism of cerebral ischemic stroke.
OBJECTIVE: To establish animal models of cerebral ischemic stroke with the photothrombosis model, and compare the infarct volume and behaviors of difference genders of mice after ischemic stroke.
METHODS: Totally 62 Kunming mice were included in this study, 6 of them were used for the pathology detection, 16 of them for the measurement of infarction volume, and 40 for behavioral test. In the pathology detection, 6 mice were equally divided into sham group and model group; in the measurement of infarction volume, 16 mice were equally divided into female model group and male model group; in the behavioral test, 40 mice were equally divided into female model group, female sham group, male model group and male sham group. In the model groups (female model group and male model group), focal cerebral ischemic stroke model was established
with the photothrombosis method; in the sham groups (female sham group and male sham group), mice were not injected with rose Bengal dye.
RESULTS AND CONCLUSION: Nissl staining found neuronal degeneration, necrosis, and vacuolar pathology at the center of infarction loci in the model groups. Fluoro-Jade C staining shown neuronal degeneration at the center of infarction loci in the model groups. Infarct volume in female group was significantly smaller than that in male group. The motor function impairment in the model group was significantly greater than that in sham group, and female group showed less impairment of motor function than male group. These results suggest that the animal model of ischemic stroke can be successfully established with the phtothrombosis method. Male animals suffer more neurological damage from ischemic stroke than female under the same condition.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Ma Jun-ning, Gao Jun-wei, Hou Bo-ru, Ren Hai-jun, Liu Ji-xing, Chen Si-hua, Yan Gui-zhong. Establishing an animal model of ischemic stroke with photothrombosis method[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(49): 7951-7957.

Figures/Tables 6

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Establishing an animal model of ischemic stroke with photothrombosis method

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