Effects of acupuncturing Changqiang acupoint on the learning and memory function and gamma-aminobutyric acid type A receptor alpha1 subunit expression in the hippocampal CA1 region of the fragile X mental retardation 1 located on X chromosome knockout mice

doi:10.3969/j.issn.2095-4344.2015.49.016

Abstract

Abstract:

BACKGROUND: γ-Aminobutyric acid type A receptor α1 subunit expression was lower in the hippocampus of mice of fragile X mental retardation 1 (FMR1) gene located on the X chromosome knockout than in wild-type mice.
OBJECTIVE: To study the effect of acupuncturing Changqiang (DU1) point on the learning and memory function and γ-aminobutyric acid type A receptor α1 subunit expression in the hippocampal CA1 region of the FMR1 located on X chromosome knockout mice.

METHODS: There were five male and five female homozygous mice of FMR1 located on X chromosome knockout. One female and one male were housed in a cage. Their offspring that was identified as homozygous mice of FMR1 located on X chromosome knockout were randomly divided into model group, Changqiang group, and non-acupuncture group (n=10). An additional 10 wild-type mice were considered as blank group. Mice in the Changqiang group received acupuncture at Changqiang acupoint for 1 minute by mild reinforcing and attenuating method, and lifting and inserting method, at the frequency of 160-200 times/minute, once a day, for 10 consecutive days. Mice in the non-acupuncture group were acupunctured at 1 cm above the lowest point of mouse right costal arch. Mice in the model and blank groups only received analog crawl.
RESULTS AND CONCLUSION: Compared with the blank group, the escape latency in the water maze was significantly prolonged in the model group (P < 0.05). Compared with the model group, the escape latency in the water maze was significantly shorter in the Changqiang group (P < 0.05), and the γ-aminobutyric acid type A receptor α1 subunit expression in the hippocampal CA1 region was increased (P < 0.05). The escape latency in the water maze and γ-aminobutyric acid type A receptor α1 subunit expression in the hippocampal CA1 region were similar between the non-acupuncture and model groups (P > 0.05). Moreover, γ-aminobutyric acid type A receptor α1 subunit expression in the hippocampal CA1 region was negatively associated with the escape latency after acupuncturing the Changqiang acupoint (r=-0.554, P=0.001). Data suggested that acupuncture at Changqiang acupoint improved the learning and memory capabilities of mice with the FMR1 located on X chromosome knockout, and up-regulated γ-aminobutyric acid type A receptor α1 subunit expression in the hippocampal CA1 region.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

CLC Number:

R318

Wu Qiang, Chen Li-yun, Zhang Xue-jun. Effects of acupuncturing Changqiang acupoint on the learning and memory function and gamma-aminobutyric acid type A receptor alpha1 subunit expression in the hippocampal CA1 region of the fragile X mental retardation 1 located on X chromosome knockout mice [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(49): 7964-7968.

Figures/Tables 3

References

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