Matrix-metalloproteinase-9 expression in focal cerebral infarction models experiencing hemorrhagic transformation

doi:10.3969/j.issn.2095-4344.2015.49.017

Abstract

Abstract:

BACKGROUND: Theoretically, hemorrhagic transformation can appear in any patients with cerebral infarction, and occurs in any process of natural evolution of cerebral infarction. Therefore, to further study the pathogenesis of hemorrhagic transformation after infarction from the angle of ischemia time and reperfusion can provide theoretical evidence for preventing and treating hemorrhagic transformation after infarction in the clinic.
OBJECTIVE: To observe matrix-metalloproteinase-9 expression in rats with persistent infarction and cerebral ischemia-reperfusion.
METHODS: Healthy male adult Wistar rats were randomly assigned to three groups: sham surgery group, ischemia-reperfusion group and persistent infarction group. Suture method was used to establish models of middle cerebral artery occlusion. In the ischemia-reperfusion group, suture was pulled out at 4.5, 6, 12, 24 and 48 hours after ischemia and reperfusion were conducted for 24 hours. In the sham surgery group, the operation was the same as the ischemia-reperfusion group, but suture was not inserted. In the persistent infarction group,

models of middle cerebral artery occlusion were generated, but the suture was not pulled out. Whether the models were successful or not was evaluated at 2 hours after the surgery. Neurological disorders were scored at corresponding time points after the surgery. 2,3,5-Triphenyltetrazolium chloride staining was utilized to observe hemorrhagic transformation in brain tissue. Immunohistochemistry was applied to determine matrix-metalloproteinase-9 expression.
RESULTS AND CONCLUSION: Within 4.5 hours after cerebral infarction, neurological score of blood recanalization was obviously elevated. 6 hours later, especially, 12 hours later, blood recanalization aggravated neurologic impairment. In the ischemia-reperfusion group, neurological score gradually decreased with prolonged time of ischemia. At 48 hours of ischemia, neurological score was lowest. 2,3,5-Triphenyltetrazolium chloride staining revealed that at 12, 24 and 48 hours after ischemia, hemorrhagic transformation appeared to different degrees in rats. Matrix-metalloproteinase-9 as a negative factor participates in reperfusion injury and hemorrhagic transformation, aggravated ischemia-reperfusion injury and promoted the onset of hemorrhagic transformation.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

CLC Number:

R318

Xiao Jie, Li Xiao-gang. Matrix-metalloproteinase-9 expression in focal cerebral infarction models experiencing hemorrhagic transformation [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(49): 7969-7975.

Figures/Tables 4

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