Establishment of an asthma model in Balb/c and DO11.10 mice

doi:10.3969/j.issn.2095-4344.2015.49.008

Abstract

Abstract:

BACKGROUND: The incidence of asthma increases year by year. Therefore, establishment and improvement of optimized animal models of asthma are important for the investigation of asthma.
OBJECTIVE: To induce asthma method in Balb/c and DO11.10 mice and to compare their difference.
METHODS: A total of 24 Balb/c mice were equally and randomly divided into groups A-D and 24 DO11.10 mice were equally divided into groups E-H. Mice in the groups A and E were induced to sensitization using PBS on day 1, and atomization using PBS on days 8-11. Mice in the groups B and F were induced to sensitization using ovalbumin on day 1, and atomization using ovalbumin on days 8-11. Mice in the groups C and G were induced to sensitization using PBS on days 1, 7 and 14, and atomization by PBS on days 22-25. Mice in the groups D and H were induced to sensitization using ovalbumin on days 1, 7 and 14, and atomization using ovalbumin on days 22-25.
RESULTS AND CONCLUSION: One time of sensitization and four times of atomization only caused mild inflammatory reaction in the lungs in Balb/c mice. Three times of sensitization and 4 times of atomization caused clear airway hyperreactivity, inflammatory cell infiltration in lung, increased mucus secretion in bronchial goblet epithelium in Balb/c mice. Simultaneously, the number of inflammatory cells and eosinophils significantly increased in bronchoalveolar lavage fluid. Th2 type cytokine levels also increased in the bronchoalveolar lavage fluid. In DO11.10 mice, one time of sensitization and four times of atomization induced significant inflammatory response. Specially, the number of neutrophils obviously increased in the bronchoalveolar lavage fluid. However, after three times of sensitization and four times of atomization, inflammatory reaction significantly decreased in DO11.10 mice. These findings indicate that asthma model could be successfully induced with ovalbum in both Balb/c and DO11.10 mice. There were some differences in the induction and pathological features for asthma model between the two species of mice.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

CLC Number:

R318

Deng Xue-quan, Yao Yin, Guo Jie-bo, Gao Wen-xiang, Sun Yue-qi, Fu Qing-ling. Establishment of an asthma model in Balb/c and DO11.10 mice[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(49): 7914-7919.

Figures/Tables 5

References

[1] Kupczyk M, Wenzel S. U.S. and European severe asthma cohorts: what can they teach us about severe asthma? J Intern Med. 2012;272(2):121-132.

[2] Galli SJ, Tsai M, Piliponsky AM. The development of allergic inflammation. Nature. 2008;454(7203):445-454.

[3] Papaioannou AI, Kostikas K, Zervas E, et al. Control of asthma in real life: still a valuable goal? Eur Respir Rev. 2015;24(136): 361-369.

[4] Fahy JV. Type 2 inflammation in asthma--present in most, absent in many. Nat Rev Immunol. 2015;15(1):57-65.

[5] 施焕中.正确认识和合理应用支气管哮喘的动物模型[J].中华结核和呼吸杂志,2005,28(11):749-750.

[6] Sun YQ, Deng MX, He J, et al. Human pluripotent stem cell-derived mesenchymal stem cells prevent allergic airway inflammation in mice. Stem Cells. 2012;30(12): 2692-2699.

[7] Chen ZG, Zhang TT, Li HT, et al. Neutralization of TSLP inhibits airway remodeling in a murine model of allergic asthma induced by chronic exposure to house dust mite. PLoS One. 2013;8(1):e51268.

[8] Kips JC, Anderson GP, Fredberg JJ, et al. Murine models of asthma. Eur Respir J. 2003;22(2):374-382.

[9] Boverhof DR, Billington R, Gollapudi BB, et al. Respiratory sensitization and allergy: current research approaches and needs. Toxicol Appl Pharmacol. 2008;226(1):1-13.

[10] Finkelman FD, Wills-Karp M. Usefulness and optimization of mouse models of allergic airway disease. J Allergy Clin Immunol. 2008;121(3):603-606.

[11] Bullone M, Lavoie JP. Asthma "of horses and men"--how can equine heaves help us better understand human asthma immunopathology and its functional consequences? Mol Immunol. 2015;66(1):97-105.

[12] Marcelino MY, Fuoco NL, de Faria CA, et al. Animal models in chronic obstructive pulmonary disease-an overview. Exp Lung Res. 2014;40(6):259-271.

[13] Fricker M, Deane A, Hansbro PM. Animal models of chronic obstructive pulmonary disease. Expert Opin Drug Discov. 2014;9(6):629-645.

[14] Pérez-Rial S, Girón-Martínez Á, Peces-Barba G. Animal models of chronic obstructive pulmonary disease. Arch Bronconeumol. 2015;51(3):121-127.

[15] Mullane K, Williams M. Animal models of asthma: reprise or reboot? Biochem Pharmacol. 2014;87(1):131-139.

[16] Wright D, Sharma P, Ryu MH, et al. Models to study airway smooth muscle contraction in vivo, ex vivo and in vitro: implications in understanding asthma. Pulm Pharmacol Ther. 2013;26(1):24-36.

[17] Nakajima H. Murine asthma model. Arerugi. 2010;59(11): 1539-1545.

[18] Chen T, Xiao L, Zhu L, et al. Anti-Asthmatic Effects of Ginsenoside Rb1 in a Mouse Model of Allergic Asthma Through Relegating Th1/Th2. Inflammation. 2015;38(5): 1814-1822.

[19] Guest IC, Sell S. Bronchial lesions of mouse model of asthma are preceded by immune complex vasculitis and induced bronchial associated lymphoid tissue (iBALT). Lab Invest. 2015;95(8):886-902.

[20] Nie H, Yang Q, Zhang G, et al. Invariant NKT cells act as an adjuvant to enhance Th2 inflammatory response in an OVA-induced mouse model of asthma. PLoS One. 2015; 10(4): e0119901.

[21] [Zhang R, Kubo M, Murakami I,et al. l-Arginine administration attenuates airway inflammation by altering l-arginine metabolism in an NC/Nga mouse model of asthma. J Clin Biochem Nutr. 2015;56(3):201-207.

[22] Hegazy AN, Klein C. Ex vivo priming of CD4 T cells converts immunological tolerance into effective antitumor immunity in a murine model of acute lymphoblastic leukemia. Leukemia. 2008;22(11):2070-2079.

[23] Nakagome K, Matsushita S, Nagata M. Neutrophilic inflammation in severe asthma. Int Arch Allergy Immunol. 2012; 158 Suppl 1:96-102.

[24] 韩灵,孙悦奇,付清玲,等.呼吸道变应性炎性反应小鼠模型的建立[J].中华耳鼻咽喉头颈外科杂志,2013,48(3):224-228.

[25] Gueders MM, Paulissen G, Crahay C, et al. Mouse models of asthma: a comparison between C57BL/6 and BALB/c strains regarding bronchial responsiveness, inflammation, and cytokine production. Inflamm Res. 2009;58(12):845-854.

[26] Zhang Y, Zhang L, Wu J, et al. Heme oxygenase-1 exerts a protective role in ovalbumin-induced neutrophilic airway inflammation by inhibiting Th17 cell-mediated immune response. J Biol Chem. 2013;288(48):34612-34626.

[27] Takagi R, Kawano M, Nakagome K, et al. Wogonin attenuates ovalbumin antigen-induced neutrophilic airway inflammation by inhibiting th17 differentiation. Int J Inflam. 2014;2014:571508.