Behavioral and morphological changes of different spinal cord injury rat models

doi:10.3969/j.issn.2095-4344.2015.49.009

Abstract

Abstract:

BACKGROUND: Complete spinal cord transection models include cut-type injury and excision-type injury models. It is necessary to compare and discuss these two models, in order to select more appropriate animal models for studying spinal cord injury and repair.
OBJECTIVE: To analyze the behavioral and pathological changes of different spinal cord injury models and to select the animal models of spinal cord injury which are more suitable for studying regeneration and treatment of injured spinal cord.
METHODS: One hundred and sixty Sprague-Dawley rats were randomly divided into two groups to establish cut-type and excision-type spinal cord injury models. At 1, 2, 4, 8, 10, 20, 30 and 50 weeks after operation, rat hindlimb locomotor skills were evaluated using BBB scores; histochemical staining and immunohistochemical staining were conducted to observe the residual fibers in injured area; at 4, 10, 26 and 52 weeks after operation, BDA-FITC tracer agent was respectively injected into the motor area of the cerebral cortex and cervical cord of
rats, to further observe the continuity of residual fibers in injured area.
RESULTS AND CONCLUSION: The BBB scores of rat hind limbs in cut-type spinal cord injury model group from 2 to 50 weeks after operation were significantly higher than those in excision-type spinal cord injury model group (P < 0.05). In cut-type spinal cord injury model group, there were a large amount of residual tissues, neurofilaments and glial fibrillary acidic protein-positive fibers in injured area, but there were almost no residual fibers in excision-type spinal cord injury model group. The corticospinal tract could pass through the injured area in neither cut-type spinal cord injury models nor excision-type spinal cord injury models and terminated at the cranial end of the injured area. In cut-type spinal cord injury models, the spinal cord propriospinal tract re-entered into the host spinal cord tissue at the caudal end, whereas the spinal cord propriospinal tract in excision-type spinal cord injury models terminated at the cranial end of the injured area. These results demonstrate that cut-type spinal cord injury models are more suitable for screening and evaluating the regeneration effect of treatments or drugs after spinal cord injury.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

CLC Number:

R318

Song Wei, Duan Hong-mei, Rao Jia-sheng, Zhao Can, Yang Zhao-yang.

Behavioral and morphological changes of different spinal cord injury rat models

[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(49): 7920-7925.

Figures/Tables 5

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