The systematic evaluation of establishing spinal cord transection model in rats

doi:10.3969/j.issn.2095-4344.2015.40.029

Abstract

Abstract:

BACKGROUND: Establishing a highly successful, safe, reliable standard spinal cord transection model is the precondition of studying spinal cord injury repair.

OBJECTIVE: To evaluate the value of preparing spinal cord transection model in rats and the effects of laminectomy on spinal cord.

METHODS: We searched the randomized controlled trials involving rat models of spinal cord transection in the databases of PubMed, CNKI, VIP and WanFang.

RESULTS AND CONCLUSION: 11 randomized controlled trials (RCTs) met the inclusion criteria (two in English, 9 in Chinese), and a total of 394 rats were included in the study. There were significant differences in the lower limb motor function scores (BBB scores) within 1-6 weeks after injury (WMD=-12.86, 95%CI -16.10 to -9.62, P < 0.01) and electrophysiological indices within 4 weeks after injury (WMD=15.36, 95%CI 11.36 to 19.36, P < 0.01) between spinal cord hemisection group and laminectomy group. The BBB scores after 6 weeks were not significantly different between these two groups (WMD=-10.28; 95%CI -24.20 to 3.64; P=0.15). There were significant differences in the lower limb motor function scores (BBB scores) within 1-6 weeks after injury (WMD= -18.83, 95% CI -20.64 to -17.01, P < 0.01) and electrophysiological indices within 4 weeks after injury (WMD= -11.21, 95%CI -16.35 to -6.08, P < 0.01) between spinal cord transection group and laminectomy group. No significant differences were found in BBB scores (WMD= -0.00, 95%CI -0.01 to 0.01, P=1) and electrophysiological indices (WMD=0.43, 95%CI -0.35 to 1.21, P=0.28) within 4 weeks after injury between laminectomy group and normal group. There was no significant difference in the number of deaths between spinal cord hemisection group and laminectomy group (RD=0.05, 95%CI -0.03 to 0.13; P=0.26). Experimental findings indicate that spinal cord transection is a method of inducing spinal cord injury with good stability, strong replication and high survival rate. But transection accuracy, postoperative care and designation of control group remain open to question.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Spinal Cord, Spinal Cord injury, Animal Model, Laminectomy

CLC Number:

R318

Jin Xin, Zhou Bin-bin, Li Bo-lin,Yang Cheng-cheng, Zhang Hong-sheng. The systematic evaluation of establishing spinal cord transection model in rats[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(40): 6553-6560.

Figures/Tables 7

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