Effect of Ferula sinkiangensis K.M. Shen on pain threshold and Fos protein expression and astrocyte activation in the spinal cord of neuropathic pain rats

doi:10.3969/j.issn.2095-4344.2015.40.017

Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (40): 6485-6491.doi: 10.3969/j.issn.2095-4344.2015.40.017

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Effect of Ferula sinkiangensis K.M. Shen on pain threshold and Fos protein expression and astrocyte activation in the spinal cord of neuropathic pain rats

Huang Yi-fei^{1, 2}, Hu Wei¹, Li Lei³, Liu Yan-lu¹

1 Second Department of Spine Surgery, Traditional Chinese Medicine Hospital of Xinjiang Uygur Antonomous Region, Urumqi 830000, Xinjiang Uygur Antonomous Region, China 2 Postdoctoral Research Station, Traditional Chinese Medicine Hospital of Xinjiang Uygur Antonomous Region, Urumqi 830000, Xinjiang Uygur Antonomous Region, China 3 Postgraduate School of Traditional Chinese Medice Hospital of Xinjiang Uygur Antonomous Region, Urumqi 830000, Xinjiang Uygur Antonomous Region, China

Online:2015-09-30 Published:2015-09-30
Contact: Hu Wei, M.D., Associate chief physician, Second Department of Spine Surgery, Traditional Chinese Medicine Hospital of Xinjiang Uygur Antonomous Region, Urumqi 830000, Xinjiang Uygur Antonomous Region, China
About author:Huang Yi-fei, M.D., Professor, Chief physician, Second Department of Spine Surgery, Traditional Chinese Medicine Hospital of Xinjiang Uygur Antonomous Region, Urumqi 830000, Xinjiang Uygur Antonomous Region, China; Postdoctoral Research Station, Traditional Chinese Medicine Hospital of Xinjiang Uygur Antonomous Region, Urumqi 830000, Xinjiang Uygur Antonomous Region, China
Supported by:
the Natural Science Foundation of Xinjiang Uygur Antonomous Region of China, No. 201233146-17

Abstract

Abstract:

BACKGROUND: Ferula sinkiangensis K.M. Shen is composed of volatile oil, resin and gum that have the anti-inflammatory, anti-allergic, antispasmodic and analgesic effects. But its analgesic mechanism is unclear.

OBJECTIVE: To observe the effect of Ferula sinkiangensis K.M. Shen on heat pain, mechanical pain, Fos protein expression and astrocyte activation in spinal cord of rats with neuropathic pain.

METHODS: Eighty adult Sprague-Dawley rat models of chronic sciatic nerve injury were randomly divided into five groups and then intragastically administered Ferula sinkiangensis K.M. Shen at low, moderate and high doses (0.075, 0.15, 0.30 g/kg), celecoxib or physiological saline. Heat pain and mechanical pain were measured at 1 day before operation and at 1, 2, 3, 5, 7, 14 days after operation. The spinal cord tissue at S₄_-₅ segments was harvested and Fos protein expression and astrocyte activation in the spinal cord of rats were observed by immunohistochemical staining method.

RESULTS AND CONCLUSION: After 1 and 5 days of medication, behavioral pain scores of rats in the low-, moderate-, and high-dose Ferula sinkiangensis K.M. Shen groups were significantly higher than that in the physiological saline group (P < 0.01). The largest reduction in heat pain threshold was measured in the moderate-dose Ferula sinkiangensis K.M. Shen group compared to the other groups (P < 0.01). The most significant reduction in rat mechanical pain threshold was measured in the high-dose Ferula sinkiangensis K.M. Shen group than in the other groups (P < 0.01). At each time point post-operation, the number of Fos protein-positive cells in the low-, moderate- and high-dose Ferula sinkiangensis K.M. Shen and celecoxib groups was significantly lower than that in the physiological saline group (P < 0.05); the number of Fos protein-positive cells in the moderate- and high-dose Ferula sinkiangensis K.M Shen groups was significantly higher than that in the celecoxib group (P < 0.05). At each time point post-operation, the number of astrocytes in the spinal cord tissue of rats in the high-dose Ferula sinkiangensis K.M. Shen and celecoxib groups was significantly lower than that in the physiological saline group (P < 0.05). There was significant difference in the number of astrocytes between the moderate- and high-dose Ferula sinkiangensis K.M shen groups and celecoxib group (P < 0.05). These results confirm that Ferula sinkiangensis K.M. Shen may effectively alleviate the neuropathic pain of rats, and the mechanism of which may be related to the activation of Fos protein and astrocytes in the spinal cord.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Tissue Engineering, Model, Animal, Pain, Sciatic Nerve

CLC Number:

R318

Huang Yi-fei, Hu Wei, Li Lei, Liu Yan-lu. Effect of Ferula sinkiangensis K.M. Shen on pain threshold and Fos protein expression and astrocyte activation in the spinal cord of neuropathic pain rats[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(40): 6485-6491.

Figures/Tables 3

References

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Effect of Ferula sinkiangensis K.M. Shen on pain threshold and Fos protein expression and astrocyte activation in the spinal cord of neuropathic pain rats

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