Effects of exercise preconditioning on expression of STAT3 and Caspase-3 in the heart of rats with exhaustive exercise injury

doi:10.3969/j.issn.2095-4344.2015.40.010

Abstract

Abstract:

BACKGROUND: Signal transducer and activator of transcription 3 (STAT3) and Caspase-3 are the important factors in JAK2/STAT3 signaling pathway. However, there are few studies on their role in the exercise preconditioning for myocardial protection.

OBJECTIVE: To analyze the effects of exercise preconditioning on the expression of STAT3 and Caspase-3 in the heart of rats and investigate the cardioprotective mechanism of action.

METHODS: Eighty Sprague-Dawley rats were randomly divided into control, exhaustive, exercise preconditioning, and exercise preconditioning+AG490 groups. The exercise preconditioning rat models were established by subjecting to intermittent treadmill exercise for 3 successive days. Rats in the control group were fed routinely for 3 days. Rats in the exhaustive group underwent treadmill exercise at 30 m/min until exhaustion after 3 days of routine raise. Rats in the exercise preconditioning group underwent exercise preconditioning for 3 days, and 24 hours later, they underwent treadmill exercises at the speed of 30 m/min until exhaustion. Rats in the exercise preconditioning+AG490 groups were intraperitoneally injected JAK2 inhibitor AG490 (3 mg/kg) at 10 minutes before exercise preconditioning and the remaining treatments were the same as that in the exercise preconditioning group. Myocardial ischemia and hypoxia degrees were detected by hematoxylin basic fuchsin picric acid staining method. The expression of STAT3 in the heart of rats was detected by Western blot assay and Caspase-3 by immunohistochemistry,respectively.

RESULTS AND CONCLUSION: Compared with the control group, the myocardial ischemia and hypoxia area and the expression of Caspase-3 and STAT3 in the heart of rats were obviously increased in the exhaustive group. Compared with the exhaustive group, the myocardial ischemia and hypoxia area and the expression of Caspase-3 in the heart of rats were noticeably decreased, but the expression of STAT3 in the heart of rats was significantly increased in the exercise preconditioning group. Compared with the exercise preconditioning group, the myocardial ischemia and hypoxia area and the expression of Caspase-3 in the heart of rats were remarkably increased, but the expression of STAT3 in the heart of rats was obviously decreased in the exercise preconditioning+AG490 group. These results suggest that exercise preconditioning plays its cardioprotective role through activating JAK2/STAT3 signaling pathway, increasing the expression of STAT3 and down-regulating the expression of Caspase-3 in the heart of rats, reducing the apoptosis of myocardial cells, and alleviating myocardial ischemia injury.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Sports Medcine, Caspase 3, Myocardium

CLC Number:

R318

Sun Xiao-juan. Effects of exercise preconditioning on expression of STAT3 and Caspase-3 in the heart of rats with exhaustive exercise injury [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(40): 6450-6454.

Figures/Tables 2

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