Regenerated cellulose materials for skin repair: preparation and performance

doi:10.3969/j.issn.2095-4344.2015.38.006

Abstract

Abstract:

BACKGROUND: The regenerated cellulose membrane has good moisture absorption and mechanical strength as well as good biodegradability, but its toughness is poorer. Studies have shown that the dry film of regenerated cellulose membrane has a poor elongation rate that is less than 15%.
OBJECTIVE: To prepare the compound regenerated cellulose membrane/collagen membrane, and to test its mechanical properties, swelling properties, moisture permeability and water retention.
METHODS: Using sodium periodate oxidation method, the 2,3-dialdehydecellulose/collagen membrane was prepared. Infrared spectroscopy and scanning electron microscope were used to observe the morphological structure, and test its mechanical properties, swelling properties, moisture permeability and water retention. Meanwhile, the regenerated cellulose membrane and 2,3-dialdehydecellulose membrane served as controls. Fibroblasts from logarithmic phase mouse embryos were seeded onto the 2,3-dialdehydecellulose/collagen membrane, and after 1, 3, 5 days, MTT method was used to detect cell proliferation.
RESULTS AND CONCLUSION: Infrared spectra showed that the collagen was cured to the surface of 2,3-dialdehydecellulose membrane. Under the scanning electron microscopy, the 2,3-dialdehydecellulose/ collagen membrane had loose porous structure, with a porosity of 93.4%. The mechanical properties, swelling rate and moisture permeability of the 2,3-dialdehydecellulose/collagen membrane were significantly stronger than those of the 2,3-dialdehydecellulose membrane and regenerated cellulose membrane (P < 0.05). However, there was no difference in the water retention rate among the three groups. Mouse embryonic fibroblasts tightly adhered to the surface of regenerated cellulose/collagen membrane, and presented a proliferative trend. These findings indicate that the 2,3-dialdehydecellulose/collagen membrane has good mechanical properties, swelling property, moisture permeability, water retention, and cytocompatibility.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Biocompatible Materials, Cellulose, Biological Dressings

CLC Number:

R318

Deng Li-huan. Regenerated cellulose materials for skin repair: preparation and performance[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(38): 6098-6103.

Figures/Tables 2

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