Ganglioside combined with bone marrow mesenchymal stem cells transplantation for treatment of traumatic brain injury

doi:10.3969/j.issn.2095-4344.2015.36.013

Abstract

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells can secrete neurotrophic factors in vitro, and can also be differentiated into neurons, thereby contributing to the repair of traumatic brain injury. However, the short life cycle of bone marrow mesenchymal stem cells influences their protective effects on the damaged brain tissues.

OBJECTIVE: To observe the effect of bone marrow mesenchymal stem cell transplantation combined with ganglioside in rats with traumatic brain injury.

METHODS: Sixty Wistar rats were used to make severe traumatic brain injury models using a hydraulic head injury instrument, and then randomized into three groups: 1 mL DMEM, 1 mL bone marrow mesenchymal stem cell suspension (1×10¹⁰/L), 1 mL bone marrow mesenchymal stem cell suspension (1×10¹⁰/L) combined with ganglioside solution (30 mg/kg) were injected respectively in model group, transplantation group and combined group, once a day, totally for 3 days. Neurological behavior scores were observed according to Longa method at 24 hours after modeling and at 3 days, 1, 2, 3, 4 weeks after cell transplantation. At 3 days after cell transplantation, RT-PCR and western blot assay were employed to detect aquaporin 4 mRNA and protein expressions. At 1 week after transplantation, hematoxylin-eosin staining was performed for pathological observation of the damaged brain tissues.

RESULTS AND CONCLUSION: At 3 days, 1, 2, 3, 4 weeks after cell transplantation, the neurological behavior scores were ranked as follows: combined group < transplantation group < model group (P < 0.05). At 3 days after cell transplantation, the mRNA and protein expression of aquaporin 4 was ranked as follows: model group > transplantation group > combined group (P < 0.05). Hematoxylin-eosin staining showed that the recovery of brain tissue was better in the combined group than the model and transplantation groups (P < 0.05). These findings indicate that bone marrow mesenchymal stem cells combined with ganglioside can significantly improve the neurological behavior function of rats with traumatic brain injury.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

CLC Number:

R394.2

Huang Yong-hui. Ganglioside combined with bone marrow mesenchymal stem cells transplantation for treatment of traumatic brain injury [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(36): 5811-5815.

Figures/Tables 3

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