Isolation, culture and in vitro proliferation of breast cancer stem cells after different cycles of neoadjuvant chemotherapy

doi:10.3969/j.issn.2095-4344.2015.36.012

Abstract

Abstract:

BACKGROUND: Whether there are breast cancer stem cell microspheres in the breast cancer tissues and whether these microspheres have an impact on isolation and culture of breast cancer stem cells after different cycles of neoadjuvant chemotherapy are still unclear.

OBJECTIVE: To explore the proliferation and differentiation of breast cancer stem cells in breast cancer tissues after different cycles of neoadjuvant chemotherapy.

METHODS: Breast cancer stem cell microspheres were isolated from the breast cancer tissues after different cycles of neoadjuvant chemotherapy to drawn a cell growth curve. Immunocytochemical method was used to detect ALDH1 expression.

RESULTS AND CONCLUSION: Microspheres could be obtained from the specimens of neoadjuvant chemotherapy for two, three and four cycles rather than one cycle. At 3 days prior to culture, there was no difference in the number of cells isolated after two- and three-cycle neoadjuvant chemotherapy; but after 3 days, the cells from the three-cycle neoadjuvant chemotherapy proliferated faster than those from the two-cycle neoadjuvant chemotherapy; after 6 days, the cell growth curve of two-cycle neoadjuvant chemotherapy was in the plateau stage, and the proliferation of cells from the three-cycle neoadjuvant chemotherapy showed a rapid increase trend. The positive expression of ALDH1 in the microspheres from the three-cycle neoadjuvant chemotherapy was higher than that from the two-cycle neoadjuvant chemotherapy. These findings indicate that breast cancer stem cells from the specimens of two- and three-cycle neoadjuvant chemotherapy both have proliferation and differentiation potentials, and the specimens of three-cycle neoadjuvant chemotherapy or above are preferred.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Breast Neoplasms, Antineoplastic Combined Chemotherapy Protocols, Cell Proliferation

CLC Number:

R394.2

Liu Wei, Wei Dong-dong, Han Li-jie. Isolation, culture and in vitro proliferation of breast cancer stem cells after different cycles of neoadjuvant chemotherapy [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(36): 5806-5810.

Figures/Tables 2

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