The homing of bone marrow mesenchymal stem cells transplanted via the trachea into rats exposed to silica dust

doi:10.3969/j.issn.2095-4344.2015.23.018

Abstract

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells transplantation via the trachea can relieve the lung injury of rats exposed to silica dust, but their distribution and migration in vivo is still unclear.
OBJECTIVE: To investigate the distribution and homing of bone marrow mesenchymal stem cells transplanted via the trachea into rats exposed to silica dust.
METHODS: Bone marrow mesenchymal stem cells from Sprague-Dawley rats were isolated through bone marrow adherent method and transfected with lentivirus carrying enhanced green fluorescent protein gene (Lv-eGFP). Trypan blue staining and cell counting kit-8 were applied to assay the viability and proliferation of the transfected and untransfected cells. Sprague-Dawley rats, SPF level, were randomized into control group and silica dust exposure group. Rats in the two groups were respectively injected via the trachea with 1 mL of sterile
silica dust suspensions (40 g/L) and 1 mL of normal saline. At 2 days after modeling, 2.2×106 transfected bone marrow mesenchymal stem cells were injected via the trachea into the rats of control group and silica dust exposure group. Rats were killed at weeks 1, 2, 3, 4 after transplantation, and the distribution and intensity of green fluorescence in the lung, heart, liver, spleen, kidney, and brain tissue were observed under the fluorescence microscopy by frozen sections and analyzed using imaging analysis software.
RESULTS AND CONCLUSION: When the multiplicity of infection was 50, there were no significant differences between the viability and proliferation activity of the transfected and untransfected cells (P > 0.05). After transplantation of transfected bone marrow mesenchymal stem cells, strong green fluorescence was observed widely in the lung, especially around the bronchus and blood vessels, and still obvious at the 4th week. The fluorescence of other organs also could be observed at the 1st week. It was strong and wide in the liver, spleen and heart, while faint and less in the kidney and brain, and all reduced with time. It shows bone marrow mesenchymal stem cells transplanted via the trachea into rats exposed to silica dust can be homing to the injured lung of rats.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cell Transplantation, Lung, Drug Administration Routes

CLC Number:

R394.2

Huang Ming, Zhou Yong-mei, Li Bin, Wu Qi-feng, Zhu Yu-feng, Liang Wei-hui. The homing of bone marrow mesenchymal stem cells transplanted via the trachea into rats exposed to silica dust [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(23): 3711-3715.

Figures/Tables 4

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