Abstract:

BACKGROUND: Human umbilical cord mesenchymal stem cells (hUC-MSCs) can obviously relieve liver cirrhosis, and thereby repair liver injury. However, the molecular mechanism of hUC-MSCs therapy for liver cirrhosis is limited at present, and especially the non-coding RNA regulation of hepatic gene changes has not been detailed.
OBJECTIVE: To investigate the changes of microRNA after hUC-MSCs therapy in rats with liver cirrhosis.
METHODS: Liver cirrhosis models were established in rats using carbon tetrachloride subcutaneous injection 
plus oral administration of alcohol. At 8 weeks after modeling, hUC-MSCs were injected via the tail vein once a week for 4 consecutive weeks. At 1 week after the last injection, rat liver tissues were collected for paraffin embedding. Liver RNA was extracted for gene chip analysis. Blood samples were collected and analyzed using an automatic biochemical analyzer to detect the changes of liver function.
RESULTS AND CONCLUSION: Alanine aminotransferase, aspartate aminotransferase and gamma-glutamyl transpeptidase were improved significantly after hUC-MSCs therapy. Fat lesions and necrosis of hepatocytes were significantly reduced. MicroRNA expression microarray hybridization analysis and PCR results showed that rno-miR-369-5p, rno-miR-3584-5p and rno-miR-153* were down-regulated during modeling and increased after hUC-MSCs therapy. And rno-miR-93, rno-miR-199a-3p, rno-miR-195, rno-let-7a and rno-miR-19a were firstly up-regulated in the process of modeling and then down-regulated obviously after hUC-MSCs therapy. These results suggest that hUC-MSCs may reverse liver cirrhosis and liver cell damage through up-regulation of rno-miR-369-5p, rno-miR-3584-5p and rno-miR-153*, and down-regulation of rno-miR-93, rno-miR-199a-3p, rno-miR-195, rno-let-7a and rno-miR-19a.  

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

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