Expression of Indian hedgehog protein and Runt related transcription factor 2 in rats with osteoarthritis caused by anterior cruciate ligament transection

doi:10.3969/j.issn.2095-4344.2015.18.006

Abstract

Abstract:

BACKGROUND: Indian hedgehog homolog (Ihh) protein and its signal protein Gli1, as well as Runt related transcription factor 2 (Runx2) are closely related to the pathogenesis of osteoarthritis. The increased expression of these factors is one of the major causes of degenerative joint changes.
OBJECTIVE: To explore the roles of Ihh, Gli1 and Runx2 in the development of osteoarthritis.
METHODS: Thirty Sprague-Dawley rats were randomly divided into two groups: control group (n=10) and model group (n=20). In the model group, rats received unilateral anterior cruciate ligament transection, to establish osteoarthritis model. Ten experimental rats were killed at 4 and 12 weeks after surgery respectively. Another 10 rats received unilateral knee arthrotomy as pseudo-operation controls and 10 pseudo-operation rats were killed at 12 weeks after surgery.
RESULTS AND CONCLUSION: In the model group, cartilage degeneration was obvious at 4 weeks and became severer at 12 weeks after anterior cruciate ligament transection operation. Expression levels of Ihh, Gli1 and Runx2 in the cartilage were increased significantly at 4 weeks after operation, but decreased at 12 weeks after operation. Experimental findings indicate that Ihh, Gli1 and Runx2 play important roles in the development of osteoarthritis, and their expression levels are significantly increased at early stage of osteoarthritis, which can be regarded as the indicators in the prophylaxis and treatment research of osteoarthritis.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: Osteoarthritis, Cartilage, Anterior Cruciate Ligament, Cartilage, Articular

CLC Number:

R318

Sun Yi-song, Yao Yun-feng, Fang Dong, Jing Jue-hua. Expression of Indian hedgehog protein and Runt related transcription factor 2 in rats with osteoarthritis caused by anterior cruciate ligament transection [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(18): 2820-2821.

Figures/Tables 3

References

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