Bone marrow mesenchymal stem cell transplantation for treatment of cis-platinum-induced ovarian damage

doi:10.3969/j.issn.2095-4344.2015.10.022

Abstract

Abstract:

BACKGROUND: Chemotherapy drugs can damage the ovarian function in women of childbearing age, and even lead to premature ovarian failure. Therefore, to improve and restore the ovarian function in patients has become an important issue.

OBJECTIVE: To explore the therapeutic effect and feasibility of bone marrow mesenchymal stem cell therapy against chemotherapy-induced ovarian damage．

METHODS: Rat models of chemotherapy-induced premature ovarian failure were established, and injected with PKH26-labeled bone marrow mesenchymal stem cells. At 15, 30, 45, 60 days after cell transplantation, five rats were selected respectively to detect follicle-stimulating hormone and estradiol levels, and then, the rats were killed to take the right ovary for pathological examination. The number of ovarian follicles was detected under light microscope. At 30 days after cell transplantation, another two rats were selected to mate with male rats to observe the difference in the reproductive activity.

RESULTS AND CONCLUSION: Four of 22 rats (18%) gradually recovered their estrous cycle after cell transplantation, with the decreased follicle-stimulating hormone level and increased estradiol level. Moreover, the number of follicles was reduced. All of these indicated that the ability to have children in rats was not damaged. These experimental findings suggest that bone marrow mesenchymal stem cells can partially improve the ovarian function of rats under chemotherapy.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Ovarian Diseases, Cisplatin, Bone Marrow, Mesenchymal Stem Cell Transplantation

CLC Number:

R394.2

Ye Xiao-feng, He Yuan-li, Fu Xia-fei, Wang Xue-feng. Bone marrow mesenchymal stem cell transplantation for treatment of cis-platinum-induced ovarian damage[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(10): 1597-1602.

Figures/Tables 7

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