DCLK1+/Ki67- cell morphology and distribution in colorectal cancer

doi:10.3969/j.issn.2095-4344.2015.10.018

Abstract

Abstract:

BACKGROUND: DCLK1 is a transmembrane microtubule-associated kinase in neurons after mitotic division, which may be the intestinal cancer stem cell marker.

OBJECTIVE:To observe the expression and pathological significance of DCLK1 and Ki67 in colorectal cancer.

METHODS: Expression of Ki67 and DCLK1 in 150 cases of colorectal cancer tissues was detected by immunohistochemical method in contrast to normal colorectal mucosa, para-carcinoma tissue, and adenoma tissue.

RESULTS AND CONCLUSION: The expression rates of DCLK1 and Ki67 were 36.7% and 34.7% in cancer tissues, respectively, both of which were significantly higher than those in normal colorectal mucosa and adenoma. The expression of DCLK1 was associated with the location, depth of invasion, lymph node metastasis (P < 0.05), while the expression of Ki67 was just associated with the depth of invasion (P < 0.05). There was a negative correlation between the expression of DCLK1 and Ki67 (r=-0.460, P=0.000). The count of DCLK1⁺/Ki67^-cells was about 2.01% in colorectal cancer tissues, and these cells mainly distributed at the bottom of intestinal mucosa base and common duct wall. DCLK1⁺/Ki67^- cells were oval, the nuclei were large and deep-stained with prominent nucleolus, and there was rare nuclear fission and less cytoplasm. From the aspects of cell number, location, and cell morphology, DCLK1⁺/Ki67^- cells are in line with the characteristics of cancer stem cells; therefore, DCLK1⁺/Ki67^-can be used as a cancer stem cell marker of colorectal cancer.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Neoplastic Stem Cells, Colorectal Neoplasms, Ki-67 Antigen

CLC Number:

R394.2

Wang Huan, Ma Fa-ku, Liu Bin, Shi Min, Xiao Wei-ling. DCLK1⁺/Ki67^- cell morphology and distribution in colorectal cancer [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(10): 1575-1579.

Figures/Tables 4

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