Transplantation of basic fibroblast growth factor-transfected bone marrow mesenchymal stem cells in the treatment of pulmonary hypertension

doi:10.3969/j.issn.2095-4344.2015.06.017

Abstract

Abstract:

BACKGROUND: Currently, emerging stem cell therapy and gene modification technology have the potential to get a unique effect in the treatment of pulmonary hypertension.
OBJECTIVE: To investigate the effects of transplantation of basic fibroblast growth factor-transfected bone marrow mesenchymal stem cells on the hemodynamic changes of pulmonary hypertension rats.
METHODS: Bone marrow mesenchymal stem cells from male Sprague-Dawley rats aged 3 weeks were cultured and purified in vitro, and then transfected with adenovirus-medicated basic fibroblast growth factor. Sixty Sprague-Dawley rats were enrolled to prepare pulmonary hypertension models through intraperitoneal injection
of 50 mg/kg monocrotaline and then randomly assigned into three groups: model group treated with transplantation of 1 mL L-DMEM via the jugular vein, cell transplantation group treated with transplantation of 1 mL untransfected bone marrow mesenchymal stem cell suspension, and transfection group treated with transplantation of 1 mL bone marrow mesenchymal stem cell suspension transfected with basic fibroblast growth factor.
RESULTS AND CONCLUSION: After 3 weeks of treatment, there were no differences in the arterial blood pressure of the three groups (P > 0.05); however, the systolic pressure of the pulmonary artery and mean pulmonary arterial pressure were significantly lower in the transfection group than the model group and cell transplantation group
(P < 0.05). Hypertrophy index of the right ventricle had no difference among the three groups (P > 0.05). The level of plasma endothelin 1 was significantly lower in the transfection group than the model group and cell transplantation group (P < 0.05). Experimental findings suggest that the transplantation of basic fibroblast growth factor-transfected bone marrow mesenchymal stem cells can improve the hemodynamic levels in pulmonary blood vessels of pulmonary hypertension rats to protect vascular endothelial cells and antagonize vascular contraction.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Bone Marrow, Mesenchymal Stem Cell Transplantation, Fibroblast Growth Factor 2, Hypertension, Pulmonary, Hemodynamics

CLC Number:

R394.2

Song Yan-feng, Cao Jie. Transplantation of basic fibroblast growth factor-transfected bone marrow mesenchymal stem cells in the treatment of pulmonary hypertension[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(6): 918-922.

Figures/Tables 2

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